LCPME, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Nancy-Université, CNRS, Faculté de Pharmacie, 5 Rue Albert Lebrun, 54000 Nancy, France.
Appl Environ Microbiol. 2010 Dec;76(24):8019-25. doi: 10.1128/AEM.00917-10. Epub 2010 Oct 15.
Detection of specific genetic markers can rapidly identify the presence of enteric viruses in groundwater. However, comparison of stability characteristics between genetic and infectivity markers is necessary to better interpret molecular data. Human adenovirus serotype 2 (HAdV2), in conjunction with MS2 phages or GA phages, was spiked into raw groundwater microcosms. Viral stability was periodically assessed by both infectivity and real-time PCR methods. The results of this yearlong study suggest that adenoviruses have the most stable persistence profile and an ability to survive for a long time in groundwater. According to a linear regression model, infectivity reductions of HAdV2 ranged from 0.0076 log(10)/day (4°C) to 0.0279 log(10)/day (20°C) and were significantly lower than those observed for phages. No adenoviral genome degradation was observed at 4°C, and the reduction was estimated at 0.0036 log(10)/day at 20°C. Occurrence study showed that DNA of human adenoviruses could be observed in groundwater from a confined aquifer (7 of the 60 samples were positive by real-time PCR), while no fecal indicators were detected. In agreement with the persistence of genetic markers, the presence of adenoviral DNA in groundwater may be misleading in term of health risk, especially in the absence of information on the infective status.
检测特定的遗传标记可以快速识别地下水中肠道病毒的存在。然而，为了更好地解释分子数据，有必要比较遗传和感染性标记的稳定性特征。人类腺病毒血清型 2（HAdV2）与 MS2 噬菌体或 GA 噬菌体一起被添加到原地下水中的微宇宙中。通过感染性和实时 PCR 方法定期评估病毒的稳定性。这项为期一年的研究结果表明，腺病毒具有最稳定的持久性特征，并且能够在地下水中长时间存活。根据线性回归模型，HAdV2 的感染性降低范围为 0.0076 log(10)/天（4°C）至 0.0279 log(10)/天（20°C），明显低于噬菌体的观察结果。在 4°C 下未观察到腺病毒基因组降解，在 20°C 下估计减少了 0.0036 log(10)/天。发生研究表明，人类腺病毒的 DNA 可以在封闭含水层的地下水中观察到（通过实时 PCR 有 7/60 个样本呈阳性），而未检测到粪便指标。与遗传标记的持久性一致，腺病毒 DNA 存在于地下水中可能会对健康风险产生误导，尤其是在缺乏感染状态信息的情况下。
