Almeida R J, Hickman-Brenner F W, Sowers E G, Puhr N D, Farmer J J, Wachsmuth I K
Department of Parasitology and Laboratory Practice, School of Public Health, University of North Carolina, Chapel Hill 27514.
J Clin Microbiol. 1990 Jan;28(1):128-30. doi: 10.1128/jcm.28.1.128-130.1990.
To determine the pandemic potential of Vibrio cholerae, one must demonstrate both the presence of O1 antigen and the production of enterotoxin (CT). Tissue culture or enzyme-linked immunosorbent assays (ELISAs) for CT have been limited to research and reference laboratories. A kit for detecting CT by reversed passive latex agglutination is now commercially available and was used to test 168 strains of V. cholerae O1 and non-O1. When compared with the routine ELISA, the latex test was 98% accurate (86 of 88) for serogroup O1 strains and 100% accurate (80 of 80) for non-O1 strains. For both O1 and non-O1 study strains, the sensitivity of the latex agglutination test was 0.97 and the specificity was 1.00 when results were compared with ELISA results. The latex test is commercially available and has the advantages of being less complicated and less time-consuming than the ELISA.
为了确定霍乱弧菌的大流行潜力，必须证明O1抗原的存在和肠毒素（CT）的产生。用于CT的组织培养或酶联免疫吸附测定（ELISA）仅限于研究和参考实验室。一种通过反向被动乳胶凝集检测CT的试剂盒现已上市，并用于检测168株霍乱弧菌O1和非O1菌株。与常规ELISA相比，乳胶试验对O1血清群菌株的准确率为98%（88株中的86株），对非O1菌株的准确率为100%（80株中的80株）。对于O1和非O1研究菌株，与ELISA结果相比，乳胶凝集试验的灵敏度为0.97，特异性为1.00。乳胶试验已上市，具有比ELISA更简单、更省时的优点。
