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分泌的整合素配体肾胞蛋白对于非洲爪蟾的前肢形成是必需的。

The secreted integrin ligand nephronectin is necessary for forelimb formation in Xenopus tropicalis.

机构信息

Division of Developmental Biology, MRC-National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK.

出版信息

Dev Biol. 2011 Jan 15;349(2):204-12. doi: 10.1016/j.ydbio.2010.10.015. Epub 2010 Oct 23.

DOI:10.1016/j.ydbio.2010.10.015
PMID:20977901
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3021715/
Abstract

While limb regeneration has been extensively studied in amphibians, little is known about the initial events in limb formation in metamorphosing anurans. The small secreted integrin ligand nephronectin (npnt) is necessary for development of the metanephros in mouse. Although expressed in many tissues, its role in other developmental processes is not well-studied. Here we show that a transgene insertion that disrupts this gene ablates forelimb formation in Xenopus tropicalis. Our results suggest a novel role for integrin signalling in limb development, and represent the first insertional phenotype to be cloned in amphibians.

摘要

虽然肢体再生在两栖动物中得到了广泛研究，但对于变态期无尾目动物肢体形成的初始事件知之甚少。小分子分泌型整合素配体肾胞外基质蛋白（npnt）是小鼠后肾发育所必需的。尽管它在许多组织中表达，但在其他发育过程中的作用尚未得到很好的研究。在这里，我们展示了一个破坏该基因的转基因插入会使爪蟾的前肢形成缺失。我们的结果表明整合素信号在肢体发育中具有新的作用，并且是在两栖动物中克隆的第一个插入表型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/20b9ed174cc6/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/e762e07ea706/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/5f736a9dfebd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/e8a10da29473/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/188d7d67b2d2/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/75fb62d699f9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/a7034fcf75a3/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/28fcc494c37c/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/432d50372a3e/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/20b9ed174cc6/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/e762e07ea706/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/5f736a9dfebd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/e8a10da29473/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/188d7d67b2d2/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/75fb62d699f9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/a7034fcf75a3/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/28fcc494c37c/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/432d50372a3e/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85ac/3021715/20b9ed174cc6/gr8.jpg

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