微流控芯片中细胞的集成电浓缩和溶解。
Integrated electrical concentration and lysis of cells in a microfluidic chip.
出版信息
Biomicrofluidics. 2010 Oct 1;4(4):44101. doi: 10.1063/1.3496358.
Abstract
Lysing cells is an important step in the analysis of intracellular contents. Concentrating cells is often required in order to acquire adequate cells for lysis. This work presents an integrated concentration and lysis of mammalian cells in a constriction microchannel using dc-biased ac electric fields. By adjusting the dc component, the electrokinetic cell motion can be precisely controlled, leading to an easy switch between concentration and lysis of red blood cells in the channel constriction. These two operations are also used in conjunction to demonstrate a continuous concentration and separation of leukemia cells from red blood cells in the same microchannel. The observed cell behaviors agree reasonably with the simulation results.
摘要
细胞裂解是分析细胞内物质的重要步骤。为了获得足够的细胞进行裂解，通常需要浓缩细胞。本工作提出了一种在直流偏置交流电场中通过收缩微通道来浓缩和裂解哺乳动物细胞的方法。通过调节直流成分，可以精确控制电动细胞运动，从而可以轻松地在通道收缩处实现红细胞的浓缩和裂解之间的切换。这两种操作还可以结合使用，以在同一微通道中连续浓缩和分离白血病细胞和红细胞。观察到的细胞行为与模拟结果相当吻合。
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