Yang L, Liu Y L, Lin S Q
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing.
Yao Xue Xue Bao. 1990;25(11):840-8.
Ten flavonoids isolated from six species of Glycyrrhiza root were analysed by reversed phase HPLC. A column packed with Partisil 5 ODS-3 and gradient elution by using different percentage of methanol (B) in water-glacial acetic acid (97:3 V/V) (A) were used to separate licochalcone A, isoschaftoside, schaftoside, liquiritin, isoviolanthin, violanthin, ononin, isoliquiritin, 4', 7-dihydroxyflavone and licoflavone A. Nine Glycyrrhiza samples of six species were analysed to determine their composition and content of the flavonoids. The results showed that the composition and content of the flavonoids of different Glycyrrhiza species were different as well as the same species but collected in different places.
采用反相高效液相色谱法对从6种甘草根中分离得到的10种黄酮类化合物进行了分析。使用填充有Partisil 5 ODS - 3的色谱柱，通过在水 - 冰醋酸（97:3 V/V）（A）中使用不同比例的甲醇（B）进行梯度洗脱，以分离甘草查尔酮A、异荭草苷、荭草苷、甘草苷、异紫花前胡苷、紫花前胡苷、芒柄花苷、异甘草苷、4',7 - 二羟基黄酮和甘草黄酮A。对6种甘草的9个样品进行分析，以确定其黄酮类化合物的组成和含量。结果表明，不同甘草品种以及同一品种但采集于不同地点的甘草中黄酮类化合物的组成和含量均有所不同。
