Zhan X C, Jones D A, Kerr A
Department of Plant Pathology, University of Adelaide, Osmond, South Australia.
Plant Mol Biol. 1990 May;14(5):785-92. doi: 10.1007/BF00016511.
Root induction on flax (Linum usitatissimum L.) cotyledon explants by Agrobacterium rhizogenes strain 1855 is markedly increased by co-inoculation with disarmed A. tumefaciens strain LBA 4404 containing a plasmid carrying the tzs gene of pTiC58. Most of the roots (estimated to be more than 90%) were transformed. This effect is most likely due to the secretion of trans-zeatin by A. tumefaciens stimulating the division of plant cells making them more receptive to transformation by A. rhizogenes, although other explanations are possible. This observation supports the idea that the tzs gene, although not essential for transformation, may promote transformation. An obvious application for genetic engineering experiments involving transformation by A. rhizogenes, is to include a vir-induced tzs gene in the transformation system to help maximize transformation efficiency.
发根农杆菌1855菌株对亚麻（Linum usitatissimum L.）子叶外植体的根诱导作用，通过与携带pTiC58的tzs基因的质粒的无致病力根癌农杆菌LBA 4404共接种而显著增强。大多数根（估计超过90%）被转化。这种效应很可能是由于根癌农杆菌分泌反式玉米素刺激植物细胞分裂，使它们更容易被发根农杆菌转化，尽管也可能有其他解释。这一观察结果支持了tzs基因虽不是转化所必需的，但可能促进转化的观点。对于涉及发根农杆菌转化的基因工程实验，一个明显的应用是在转化系统中加入一个vir诱导的tzs基因，以帮助最大限度地提高转化效率。
