Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, China.
ACS Nano. 2010 Nov 23;4(11):6439-48. doi: 10.1021/nn101373r. Epub 2010 Oct 28.
Understanding the interaction mechanisms between nanomaterials and biological cells is important for the control and manipulation of these interactions for biomedical applications. In this study, we investigated the cellular effects of gold nanoparticles (AuNPs) on the differentiation of mesenchymal stem cells (MSCs) and the associated molecular mechanisms. The results showed that AuNPs promoted the differentiation of MSCs toward osteoblast cells over adipocyte cells by inducing an enhanced osteogenic transcriptional profile and an attenuated adipogenic transcriptional profile. AuNPs exerted the effects by interacting with the cell membrane and binding with proteins in the cytoplasm, causing mechanical stress on the MSCs to activate p38 mitogen-activated protein kinase pathway (MAPK) signaling pathway, which regulates the expression of relevant genes to induce osteogenic differentiation and inhibit adipogenic differentiation.
了解纳米材料与生物细胞之间的相互作用机制对于控制和操纵这些相互作用以应用于生物医学非常重要。在这项研究中，我们研究了金纳米粒子（AuNPs）对间充质干细胞（MSCs）分化的细胞效应及其相关的分子机制。结果表明，AuNPs 通过诱导增强的成骨转录谱和减弱的成脂转录谱，促进 MSCs 向成骨细胞分化而不是脂肪细胞分化。AuNPs 通过与细胞膜相互作用并与细胞质中的蛋白质结合，对 MSCs 施加机械应力，激活 p38 丝裂原活化蛋白激酶途径（MAPK）信号通路，从而调节相关基因的表达，诱导成骨分化并抑制成脂分化。
