两种菜豆（菜豆属）叶绿体苯丙氨酸转运核糖核酸（tRNAs(Phe)）在次要核苷酸含量上存在差异，其相对含量在叶片发育过程中的变化。
Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAs(Phe) which differ in their minor nucleotide content.
作者信息
Pfitzinger H, Maréchal-Drouard L, Pillay D T, Weil J H, Guillemaut P
机构信息
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
出版信息
Plant Mol Biol. 1990 Jun;14(6):969-75. doi: 10.1007/BF00019393.
Bean (Phaseolus vulgaris cv. Saxa) chloroplasts contain two tRNA(Phe) species, namely tRNA(Phe)1 and tRNA(Phe)2. By sequence determination, we show that tRNA(Phe)2 is identical to the previously sequenced tRNA(Phe)1 except for two undermodified nucleotides. By reversed-phase chromatography analyses, we demonstrate that the relative amounts of these two chloroplast tRNAs(Phe) vary during leaf development: in etiolated leaves the undermodified tRNA(Phe)2 only represents 15% of total chloroplast tRNA(Phe), during development and greening it increases to reach 60% in 8-day-old leaves, and it then decreases to 9% in senescing leaves.
菜豆（菜豆品种Saxa）叶绿体含有两种tRNA（Phe），即tRNA（Phe）1和tRNA（Phe）2。通过序列测定，我们发现tRNA（Phe）2与先前测序的tRNA（Phe）1相同，只是有两个修饰不足的核苷酸。通过反相色谱分析，我们证明这两种叶绿体tRNA（Phe）的相对含量在叶片发育过程中会发生变化：在黄化叶片中，修饰不足的tRNA（Phe）2仅占叶绿体总tRNA（Phe）的15%，在发育和变绿过程中，它增加到8日龄叶片中的60%，然后在衰老叶片中降至9%。
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