Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.
Neurotoxicol Teratol. 2011 Mar-Apr;33(2):329-32. doi: 10.1016/j.ntt.2010.10.006. Epub 2010 Oct 28.
Environmental silver exposures are increasing due to the use of silver nanoparticles, which exert antimicrobial actions by releasing Ag+, a suspected developmental neurotoxicant. We evaluated the long-term neurochemical and behavioral effects of embryonic Ag+ exposure in zebrafish at concentrations that had no overt effects on morphological development. Exposure to 0.03, 0.1 or 0.3 μM Ag+ during the first five days post-fertilization caused elevations in both dopamine and serotonin turnover in the adult zebrafish brain without affecting basal neurotransmitter levels. Consistent with these synaptic effects, Ag+-exposed fish showed a faster acquisition of avoidance behavior in a three-chamber test apparatus, without any change in response latency or overall swimming ability. Our results indicate that Ag+ is a developmental neurotoxicant that causes persistent neurobehavioral effects, reinforcing health concerns about Ag+ released from silver nanoparticles.
由于纳米银的使用，环境中的银暴露量正在增加，纳米银通过释放银离子（一种疑似发育神经毒物）发挥抗菌作用。我们评估了胚胎期斑马鱼暴露于浓度不会对形态发育产生明显影响的银离子中的长期神经化学和行为影响。在受精后第 5 天内暴露于 0.03、0.1 或 0.3 μM 的银离子会导致成年斑马鱼大脑中的多巴胺和血清素周转率升高，而不会影响基础神经递质水平。与这些突触效应一致，暴露于银离子的鱼在三室测试仪器中表现出更快的回避行为习得，而反应潜伏期或整体游泳能力没有任何变化。我们的结果表明，银离子是一种发育神经毒物，会导致持续的神经行为影响，这加剧了对纳米银释放的银离子的健康担忧。
