Samadi Assadullah, Ababneh M Mk, Giadinis N D, Lafi S Q
Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.
Vet Med Int. 2010 Oct 28;2010:458695. doi: 10.4061/2010/458695.
Two hundred and fifty five biological samples were collected from 188 animals (81 sheep and 107 goats) during the lambing season from September 2009 to April 2010 from the Mafraq region of Jordan. Sampled animals belonged to 93 sheep and goat flocks that had abortion cases in the region. One hundred and seven (41.9%) biological samples were positive for the omp2 primers that were able to identify all Brucella species in the collected samples which were obtained from 86 aborted animals (86/188 = 45.7%). Using the B. melitensis insertion sequence 711 (IS711) primers on the 107 omp2 positive samples, only 61 confirmed to be positive for B. melitensis. These positive samples were obtained from 28 sheep and 33 goats. The prevalence rate of B. melitensis was 27.1% (51/188) among aborted animals. For differentiation between vaccine strain and field strain infection, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using PstI endonuclease enzyme was used. Vaccination with Rev-1 in the last year (OR = 2.92, CI: 1.1-7.7) and grazing at common pasture (OR = 2.78, CI: 1.05-7.36) were statistically significant (P ≤ .05) risk factors positively associated with the occurrence of brucellosis in sheep and goat flocks.
2009年9月至2010年4月产羔季节期间，从约旦马弗拉克地区的188只动物（81只绵羊和107只山羊）身上采集了255份生物样本。采样的动物来自该地区93个发生流产病例的绵羊和山羊群。107份（41.9%）生物样本对omp2引物呈阳性，该引物能够鉴定采集样本中的所有布鲁氏菌物种，这些样本来自86只流产动物（86/188 = 45.7%）。对107份omp2阳性样本使用羊种布鲁氏菌插入序列711（IS711）引物，只有61份被确认为羊种布鲁氏菌阳性。这些阳性样本来自28只绵羊和33只山羊。流产动物中羊种布鲁氏菌的患病率为27.1%（51/188）。为了区分疫苗株感染和野外株感染，使用了使用PstI内切酶的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法。去年接种Rev-1疫苗（OR = 2.92，CI：1.1 - 7.7）以及在公共牧场放牧（OR = 2.78，CI：1.05 - 7.36）是与绵羊和山羊群布鲁氏菌病发生呈正相关的统计学显著（P≤0.05）风险因素。
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