Thomas Philip, Fenech Michael
CSIRO Human Nutrition, Adelaide, SA, Australia.
Methods Mol Biol. 2011;682:235-48. doi: 10.1007/978-1-60327-409-8_17.
The Buccal Micronucleus Cytome (BMCyt) assay is a new minimally invasive system for studying DNA damage, chromosomal instability, cell death, and the regenerative potential of buccal mucosal tissue. This method is increasingly being used in molecular epidemiologic studies investigating the impact of nutrition, life-style factors, genotoxin exposure, and genotype on DNA damage and cell death. Biomarkers of this assay have been associated with increased risk for accelerated aging, cancer, and neurodegenerative diseases. This protocol describes the current established methods for buccal cell collection, slide preparation, cellular and nuclear staining, and scoring criteria.
口腔微核细胞组（BMCyt）检测是一种用于研究DNA损伤、染色体不稳定性、细胞死亡以及口腔黏膜组织再生潜能的新型微创系统。该方法越来越多地应用于分子流行病学研究，以调查营养、生活方式因素、基因毒素暴露和基因型对DNA损伤及细胞死亡的影响。此检测的生物标志物与加速衰老、癌症和神经退行性疾病风险增加相关。本方案描述了目前已确立的口腔细胞采集、玻片制备、细胞和细胞核染色以及评分标准的方法。
