Miller C C, Ziboh V A, Wong T, Fletcher M P
Department of Dermatology, University of California, Davis 95616.
J Nutr. 1990 Jan;120(1):36-44. doi: 10.1093/jn/120.1.36.
Certain dietary oils may have therapeutic potential in the treatment of inflammatory skin disorders. Presumably, the fatty acid constituents of these dietary oils exert their effects by altering the levels of cutaneous eicosanoids. Prompted by this possibility, we investigated whether supplementation of guinea pig diets with fish oil [rich in 20:5(n-3)] or borage oil [rich in 18:3(n-6)] could significantly alter epidermal levels of eicosanoids compared with control animals supplemented with olive oil. After feeding periods of 4, 8 or 12 wk, the epidermis from the animals was analyzed for: 1) fatty acid composition of individual epidermal phospholipids, 2) levels of lipoxygenase products, and 3) levels of cyclooxygenase products (prostaglandins). Our results demonstrated that the animals supplemented with dietary fish oil had elevated levels of 20:5(n-3) in epidermal phospholipids and elevated epidermal levels of 15-hydroxyeicosapentaenoic acid (15-HEPE) [the 15-lipoxygenase product of 20:5(n-3)] compared with guinea pigs fed olive oil or borage oil. Similarly, the animals supplemented with dietary borage oil had elevated levels of 20:3(n-6) [the epidermal elongase product of 18:3(n-6)] in epidermal phospholipids and elevated epidermal levels of 15-hydroxyeicosatrienoic acid [15-HETrE, the epidermal 15-lipoxygenase product of 20:3(n-6)] compared with guinea pigs fed olive oil or fish body oil. There were no significant changes in epidermal levels of prostaglandins. Both 15-HEPE and 15-HETrE have been identified as possible anti-inflammatory metabolites, and their elevated presence in the epidermis of animals fed oils rich in 20:5(n-3) or 18:3(n-6) may provide a mechanism for the beneficial effects of these oils on inflammatory conditions.
某些食用油可能在治疗炎症性皮肤病方面具有治疗潜力。据推测，这些食用油中的脂肪酸成分通过改变皮肤类二十烷酸的水平发挥作用。受此可能性的启发，我们研究了用鱼油[富含20:5(n-3)]或琉璃苣油[富含18:3(n-6)]补充豚鼠饮食是否能与补充橄榄油的对照动物相比，显著改变表皮类二十烷酸的水平。在4周、8周或12周的喂养期后，对动物的表皮进行分析：1) 单个表皮磷脂的脂肪酸组成，2) 脂氧合酶产物的水平，以及3) 环氧化酶产物（前列腺素）的水平。我们的结果表明，与喂食橄榄油或琉璃苣油的豚鼠相比，补充膳食鱼油的动物表皮磷脂中20:5(n-3)的水平升高，表皮中15-羟基二十碳五烯酸(15-HEPE)[20:5(n-3)的15-脂氧合酶产物]的水平升高。同样，与喂食橄榄油或鱼油的豚鼠相比，补充膳食琉璃苣油的动物表皮磷脂中20:3(n-6)[18:3(n-6)的表皮延长酶产物]的水平升高，表皮中15-羟基二十碳三烯酸[15-HETrE，20:3(n-6)的表皮15-脂氧合酶产物]的水平升高。前列腺素的表皮水平没有显著变化。15-HEPE和15-HETrE都已被确定为可能的抗炎代谢物，它们在喂食富含20:5(n-3)或第18:3(n-6)油的动物表皮中的升高可能为这些油对炎症状况的有益作用提供一种机制。
