Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, IL 61801, USA.
J Econ Entomol. 2010 Oct;103(5):1815-20. doi: 10.1603/ec10029.
To determine their baseline susceptibility to chlorantraniliprole, spinetoram, spinosad, and acetamiprid, oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), neonates were placed on diet cubes containing a range of concentrations of each insecticide. Mortality was assessed after 96 h. Two populations-a long-term laboratory colony from Rutgers University and a colony established in 2007 from a southwestern Illinois (Calhoun County) field population-were tested. We used probit and logit analyses to compare the responses of Calhoun colony neonates from parents reared on 'Gala' apples (Malus spp.) with those of Calhoun colony neonates from parents reared on lima bean, Phaseolus lunatus L., diet. We also compared the responses of Calhoun colony neonates with those of Rutgers colony neonates (all from parents reared on apples). LC50s (ppm in diet) for Calhoun colony progeny of adults reared on apples were 0.08, 0.06, 0.41, and 0.30, respectively, for chlorantraniliprole, spinetoram, acetamiprid, and spinosad. Parental food source (apples versus lima bean diet) did not consistently influence the concentration-mortality relationships for neonates. Based on LC50s and toxicity ratio tests, Calhoun colony neonates were slightly but significantly less susceptible to spinetoram and acetamiprid than were Rutgers colony neonates. Similarly, LC90s and toxicity ratio tests indicated that Calhoun colony neonates were slightly but significantly less susceptible to chlorantraniliprole as well. However, toxicity ratios (Calhoun/Rutgers) were low in all instances, and the highest ratio was 1.73 at LC90 for chlorantraniliprole. Overall, the two colonies responded similarly to these insecticides. Results reported here provide baseline data for future monitoring of resistance development.
为了确定东方果实蝇（Grapholita molesta（Busck））对氯虫苯甲酰胺、螺虫乙酯、多杀菌素和噻虫嗪的基础敏感性，将新孵化的幼虫置于含有一系列浓度的每种杀虫剂的饮食立方体上。96 小时后评估死亡率。测试了两个种群-来自罗格斯大学的长期实验室种群和 2007 年从伊利诺伊州西南部（卡尔霍恩县）田间种群建立的种群。我们使用概率单位和对数分析比较了在 Gala 苹果（Malus spp.）上饲养的父母饲养的卡尔霍恩种群幼虫的反应与在利马豆（Phaseolus lunatus L.）饮食上饲养的父母饲养的卡尔霍恩种群幼虫的反应。我们还比较了卡尔霍恩种群幼虫与罗格斯种群幼虫（均来自苹果饲养的父母）的反应。在苹果上饲养的成虫后代的卡尔霍恩种群幼虫的 LC50（饮食中的 ppm）分别为 0.08、0.06、0.41 和 0.30，分别为氯虫苯甲酰胺、螺虫乙酯、噻虫嗪和多杀菌素。父母的食物来源（苹果与利马豆饮食）并不一致影响幼虫的浓度-死亡率关系。基于 LC50 和毒性比测试，与罗格斯种群幼虫相比，卡尔霍恩种群幼虫对螺虫乙酯和噻虫嗪的敏感性略低，但有显著差异。同样，LC90 和毒性比测试表明，卡尔霍恩种群幼虫对氯虫苯甲酰胺的敏感性也略低。然而，在所有情况下，毒性比（卡尔霍恩/罗格斯）都很低，氯虫苯甲酰胺的最高比值为 LC90 时的 1.73。总的来说，这两个种群对这些杀虫剂的反应相似。这里报告的结果为未来监测抗药性发展提供了基线数据。
