Department of Head and Neck Surgery, Sun Yat-sen University Cancer Center, No 651 Dongfeng East Road, Guangzhou, Guangdong, People's Republic of China.
Med Oncol. 2011 Dec;28 Suppl 1:S360-6. doi: 10.1007/s12032-010-9737-3. Epub 2010 Nov 11.
COX-2 induces the proliferation and invasion of oral squamous cell carcinoma. In the present study, the role of the COX-2 gene in the tongue cancer cell proliferation and invasion was investigated. A short hairpin RNA (shRNA)method was used to knock down COX-2 gene expression and investigate the relationship between COX-2 and VEGF-C, and the role of the COX-2 gene for proliferation and invasion was also investigated in the tongue cancer cell Tca8113. COX-2 gene overexpressed in tongue cancer cell line. Suppressing the expression of COX-2 by shRNA could decrease cell proliferation comparing with control shRNA. Nevertheless, depressing COX-2 gene expression by shRNA reduced VEGF-C expression on both mRNA and protein levels. VEGF-C gene expression could be regulated by COX-2 gene. Our results suggested that COX-2 played essential roles in the proliferation and metastasis of tongue cancer, and COX-2 could serve as a potential chemotherapy target for tongue cancer.
COX-2 可诱导口腔鳞状细胞癌的增殖和侵袭。在本研究中，研究了 COX-2 基因在舌癌细胞增殖和侵袭中的作用。采用短发夹 RNA(shRNA)方法敲低 COX-2 基因表达，并研究 COX-2 与 VEGF-C 之间的关系，以及 COX-2 基因在舌癌细胞 Tca8113 中的增殖和侵袭作用。COX-2 基因在舌癌细胞系中过表达。与对照 shRNA 相比，shRNA 抑制 COX-2 的表达可降低细胞增殖。然而，shRNA 抑制 COX-2 基因表达可降低 VEGF-C 在 mRNA 和蛋白水平的表达。VEGF-C 基因的表达可受 COX-2 基因调控。我们的结果表明，COX-2 在舌癌的增殖和转移中发挥重要作用，COX-2 可作为舌癌潜在的化疗靶点。
