Shimizu M, Shirai J, Narita M, Yamane T
National Institute of Animal Health, Ibaraki, Japan.
J Clin Microbiol. 1990 Feb;28(2):201-6. doi: 10.1128/jcm.28.2.201-206.1990.
A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine astrovirus was shown to have an RNA genome, as determined by the effect of 5-iodo-2'-deoxyuridine on its replication, and five polypeptides with molecular masses of 13,000, 30,000, 31,000, 36,000, and 39,000 daltons; and it was shown to be stable to lipid solvents and heating at 50 degrees C for 30 min but somewhat labile to acid (pH 3.0). The buoyant density of the isolate determined in CsCl was 1.35 g/ml. Seroconversion to the virus was evident in the paired serum specimens obtained from pigs with diarrhea that were housed at the farm where the disease occurred. The neutralization test on serum specimens collected randomly from 128 adult pigs of eight herds revealed that 50 of the serum specimens were positive for antibody to porcine astrovirus, although there was considerable variation in the prevalence among herds, ranging from 0 to 83%. Hysterectomy-produced, colostrum-deprived, 4-day-old pigs developed mild diarrhea after oral exposure to porcine astrovirus propagated in the cell culture; and the virus was isolated again from diarrheal stool specimens.
从患有急性腹泻的猪中分离出一种细胞病变性星状病毒，该病毒在借助胰蛋白酶从猪胚胎肾中获得的已建立细胞系中培养。该病毒表现出明显的细胞病变效应，其特征为细胞肿大以及细胞质中出现细颗粒。通过5-碘-2'-脱氧尿苷对其复制的影响确定，猪星状病毒具有RNA基因组，还有分子量分别为13,000、30,000、31,000、36,000和39,000道尔顿的五种多肽；并且该病毒对脂溶剂稳定，在50℃加热30分钟也稳定，但对酸（pH 3.0）有些敏感。在氯化铯中测定的该分离株的浮力密度为1.35 g/ml。在发病猪场饲养的腹泻猪的配对血清样本中，明显出现了针对该病毒的血清转化。对从八个猪群的128头成年猪中随机采集的血清样本进行中和试验发现，50份血清样本对猪星状病毒抗体呈阳性，尽管各猪群的流行率差异很大，范围从0到83%。经子宫切除、未摄入初乳的4日龄仔猪经口接触在细胞培养物中繁殖的猪星状病毒后出现轻度腹泻；并且从腹泻粪便样本中再次分离出该病毒。
