Chakravarti Anita, Chauhan Mayank Singh, Sharma Anju, Verma Vikas
Department of Microbiology, Maulana Azad Medical College and Associated Lok Nayak Hospital, New Delhi, India.
Southeast Asian J Trop Med Public Health. 2010 Sep;41(5):1145-52.
Rotavirus gastroenteritis is a major cause of severe dehydrating diarrhea in children worldwide. Rotavirus G and P genotyping is essential for epidemiological surveillance and for better formulation of candidate rotavirus vaccines. Out of 862 diarrheal stool samples collected from hospitalized children aged < 2 years during February 2005 - March 2007, 318 (36.9%) were positive for rotavirus by ELISA. G and P genotyping was performed on 100 randomly selected positive samples using a seminested multiplex RT-PCR assay. The result of G genotyping indicates G1 (60%) was the most predominant VP7 type, followed by G2 (16%), G9 (8%) and G3 (3%). Two cases of G12 genotype were also observed. P genotypes identified were P[8] (40%) followed by P[4] (26%) and P[6] (17%). The most common G-P combinations were G1P[8] (26%), followed by G1P[4] and G1P[6]. Mixed infection involved 28% of strains. In this study the G1 and P[8] genotypes were the leading G and P types. Two cases with G12 genotype were also observed during the study.
轮状病毒肠胃炎是全球范围内儿童严重脱水腹泻的主要病因。轮状病毒G和P基因分型对于流行病学监测以及更好地制定轮状病毒候选疫苗至关重要。在2005年2月至2007年3月期间从2岁以下住院儿童中收集的862份腹泻粪便样本中，通过酶联免疫吸附测定法（ELISA）检测出318份（36.9%）轮状病毒呈阳性。使用半巢式多重逆转录聚合酶链反应（RT-PCR）分析法对100份随机选择的阳性样本进行了G和P基因分型。G基因分型结果表明，G1（60%）是最主要的VP7型，其次是G2（16%）、G9（8%）和G3（3%）。还观察到2例G12基因型病例。鉴定出的P基因型为P[8]（40%），其次是P[4]（26%）和P[6]（17%）。最常见的G-P组合是G1P[8]（26%），其次是G1P[4]和G1P[6]。混合感染涉及28%的菌株。在本研究中，G1和P[8]基因型是主要的G和P类型。研究期间还观察到2例G12基因型病例。
