Xu Gang, Yang Jing, Wang Yuan-Yuan, Peng Li-Yan, Yang Xian-Wen, Pan Zheng-Hong, Liu En-De, Li Yan, Zhao Qin-Shi
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China.
J Agric Food Chem. 2010 Dec 8;58(23):12157-61. doi: 10.1021/jf103366g. Epub 2010 Nov 12.
Salvia digitaloides, belonging to the economically and medicinally important genus Salvia, is distributed in the northwest of Yunnan Province, People's Republic of China. The roots of this plant were soaked in alcohol by local Tibetans to make a special traditional "red wine", and many local people like to drink this traditional "red wine" to strengthen physical health. To investigate the bioactive diterpenoid constituents of the roots, a detailed phytochemical study was carried out, and 13 diterpenoids including two new norditerpenoids, dihydroneotanshinlactone (1) and 16,17-dinorpisferal A (2), were isolated. Their structures were established on the basis of detailed spectroscopic analysis. In addition, computational methods were applied to validate the stereochemistry of compound 1. For the bioassay to inhibit the growth of five tumor cell lines, neotanshinlactone (3) exhibited selective cytotoxic activity toward the human breast cancer cell line SK-BR-3, while the other four tanshinones (4-7) showed significant toxicity effects against all of the tested five cell lines.
数码鼠尾草属于具有重要经济和药用价值的鼠尾草属，分布于中华人民共和国云南省西北部。当地藏族人将这种植物的根浸泡在酒精中制成一种特殊的传统“红酒”，许多当地人喜欢饮用这种传统“红酒”来强身健体。为了研究该植物根中的生物活性二萜成分，开展了详细的植物化学研究，分离得到13个二萜类化合物，包括两个新的降二萜类化合物，二氢新丹参内酯（1）和16,17-二降紫草酸A（2）。它们的结构通过详细的光谱分析得以确定。此外，应用计算方法验证了化合物1的立体化学。在对五种肿瘤细胞系生长抑制的生物活性测试中，新丹参内酯（3）对人乳腺癌细胞系SK-BR-3表现出选择性细胞毒性活性，而其他四种丹参酮（4-7）对所有测试的五种细胞系均显示出显著的毒性作用。
