Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, 2250 Alcazar Street, CSA 103, Los Angeles, CA 90033, USA.
J Dent Res. 2011 Mar;90(3):317-24. doi: 10.1177/0022034510387796. Epub 2010 Nov 12.
Although human orofacial bone-marrow-derived mesenchymal stem cells showed differentiation traits distinctly different from those of mesenchymal stem cells (MSCs) derived from long bone marrow (BMMSCs), mouse MSCs derived from orofacial bone have not been isolated due to technical difficulties, which in turn precludes the use of mouse models to study and cure orofacial diseases. In this study, we developed techniques to isolate and expand mouse orofacial bone/bone-marrow-derived MSCs (OMSCs) from mandibles and verified their MSC characteristics by single-colony formation, multi-lineage differentiation, and in vivo tissue regeneration. Activated T-lymphocytes impaired OMSCs via the Fas/Fas ligand pathway, as occurs in BMMSCs. Furthermore, we found that OMSCs are distinct from BMMSCs with respect to regulating T-lymphocyte survival and proliferation. Analysis of our data suggests that OMSCs are a unique population of MSCs and play an important role in systemic immunity.
BMMSC, bone marrow mesenchymal stem cell; HA/TCP, hydroxyapatite/tricalcium phosphate; OMSC, orofacial mesenchymal stem cell; OVX, ovariectomized.
虽然人类口腔颌骨骨髓间充质干细胞表现出与长骨髓间充质干细胞（BMMSCs）明显不同的分化特征，但由于技术困难，尚未分离出源自口腔颌骨的小鼠间充质干细胞，这反过来又妨碍了使用小鼠模型来研究和治疗口腔颌面部疾病。在这项研究中，我们开发了从下颌骨分离和扩增小鼠口腔颌骨/骨髓来源间充质干细胞（OMSCs）的技术，并通过单细胞形成、多谱系分化和体内组织再生验证了其 MSC 特征。激活的 T 淋巴细胞通过 Fas/Fas 配体途径损伤 OMSCs，就像在 BMMSCs 中一样。此外，我们发现 OMSCs 在调节 T 淋巴细胞存活和增殖方面与 BMMSCs 不同。对我们数据的分析表明，OMSCs 是间充质干细胞的一个独特群体，在全身免疫中发挥重要作用。
BMMSC，骨髓间充质干细胞；HA/TCP，羟基磷灰石/磷酸三钙；OMSC，口腔间充质干细胞；OVX，卵巢切除。
