Prabha K, Baranwal V K, Jain R K
Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India.
Indian J Virol. 2013 Sep;24(2):157-65. doi: 10.1007/s13337-013-0133-4. Epub 2013 May 11.
Present era of molecular biology is witnessing revolutionary developments in sequencing technology. This advancement has considerably influenced plant virology in the field of diagnostics and host virus interaction. Next generation high-throughput sequencing technology has made it possible to directly detect, identify and discover novel viruses in several plants in an unbiased manner without antibodies or prior knowledge of the virus sequences. Entire viral genome could be sequenced from symptomatic or asymptomatic plants through next generation sequencing of total nucleic acids including small RNAs. It provides census of viral population in a particular ecosystem or cropping system. Viral genome variability, evolution within the host and virus defence mechanism in plants can also be easily understood by massive parallel sequencing. In this article, we provide an overview of the applications of next generation sequencing technology in characterization, discovery and molecular interaction of plant viruses.
当今分子生物学时代，测序技术正经历着革命性的发展。这一进展在诊断和宿主病毒相互作用领域对植物病毒学产生了相当大的影响。新一代高通量测序技术使得在不使用抗体或无需事先了解病毒序列的情况下，能够以无偏见的方式直接检测、鉴定和发现多种植物中的新型病毒。通过对包括小RNA在内的总核酸进行新一代测序，可以从有症状或无症状的植物中对整个病毒基因组进行测序。它提供了特定生态系统或种植系统中病毒群体的普查信息。通过大规模平行测序，还可以轻松了解病毒基因组的变异性、在宿主内的进化以及植物中的病毒防御机制。在本文中,我们概述了新一代测序技术在植物病毒鉴定表征、发现及分子相互作用方面的应用。
