Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Directorate of Chemical Safety of the Food Chain, Unit of Toxins and Natural Components, Leuvensesteenweg 17, 3080 Tervuren, Belgium.
J Agric Food Chem. 2010 Dec 22;58(24):12625-33. doi: 10.1021/jf103025e. Epub 2010 Nov 18.
Cross-reactivity of antibodies in AGRAQUANT, DON EIA, VERATOX, ROSA LF-DONQ, and MYCONTROLDON designed for deoxynivalenol (DON) determination in food and feedstuffs was evaluated against nivalenol, 3-acetylDON, 15-acetylDON, de-epoxy metabolite 1 of DON, DON-3β-glucoside, T2-toxin, HT2-toxin, fusarenone X, diacetoxyscirpenol, verrucarol, and zearalenone. Cross-reactivity measurements were run in water using the 50% reduction of absorbance of the blank for ELISA kits or through direct DON determination upon using the standards of mycotoxins via ROSA LF-DONQ or MYCONTROLDON. For the tested toxin concentrations, all DON kits have low cross-reactivity toward diacetoxyscirpenol, T2-toxin, HT2-toxin, verrucarol, and zearalenone and moderate cross-reactivity toward 15-AcetylDON and fusarenone X. AGRAQUANT, DON EIA, and VERATOX kits showed high cross-reactivity in various ranking orders against DON-3-Glc, DOM-1, and 3AcDON. DON EIA showed also high cross-reactivity against nivalenol and fusarenone X. These mycotoxins could coexist in food or feedstuffs, and analytical results can be wrongly interpreted. Cross-reactivity does not allow checking the compliance with the legal norms, but it does allow an overall risk assessment for the consumers. Updating regularly the cross-reactivity evaluation of the produced batches is recommended for 3-acetylDON, nivalenol, DON-3-Glc, de-epoxy metabolite 1, and fusarenone X.
用于食品和饲料中脱氧雪腐镰刀菌烯醇（DON）测定的 AGRAQUANT、DON EIA、VERATOX、Rosa LF-DONQ 和 MYCONTROLDON 抗体的交叉反应性，针对雪腐镰刀菌烯醇、3-乙酰基 DON、15-乙酰基 DON、DON-1 去环氧代谢物、DON-3β-葡萄糖苷、T2 毒素、HT2 毒素、伏马菌素 X、二乙酰氧基麦角甾醇、疣孢青霉毒素和玉米赤霉烯酮进行了评估。交叉反应性测量是在水中进行的，使用 ELISA 试剂盒空白吸光度的 50%降低，或者通过使用 ROSA LF-DONQ 或 MYCONTROLDON 中的霉菌毒素标准直接测定 DON。对于测试的毒素浓度，所有 DON 试剂盒对二乙酰氧基麦角甾醇、T2 毒素、HT2 毒素、疣孢青霉毒素和玉米赤霉烯酮的交叉反应性较低，对 15-乙酰基 DON 和伏马菌素 X 的交叉反应性中等。AGRAQUANT、DON EIA 和 VERATOX 试剂盒在各种排序中对 DON-3-Glc、DOM-1 和 3AcDON 表现出高交叉反应性。DON EIA 对雪腐镰刀菌烯醇和伏马菌素 X 也表现出高交叉反应性。这些霉菌毒素可能同时存在于食品或饲料中，分析结果可能会被错误解释。交叉反应性不允许检查是否符合法律规范，但它允许对消费者进行全面的风险评估。建议定期更新 3-乙酰基 DON、雪腐镰刀菌烯醇、DON-3-Glc、去环氧代谢物 1 和伏马菌素 X 生产批次的交叉反应性评估。
