Niilo L
Can J Comp Med. 1978 Jul;42(3):357-63.
One hundred and fourteen strains of Clostridium perfringens, isolated from the intestinal contents of cattle, sheep, and chickens with enteritis or other disease conditions were studied for their ability to produce enterotoxin. Reversed passive hemagglutination, fluorescent antibody and immunodiffusion tests were used. On the basis of the reversed passive hemagglutination titres, supported by the other two tests, enterotoxigenicity of the strains was arbitrarily classified into two categories: highly enterotoxigenic and potentially enterotoxigenic, with 12% falling into each category. All the highly enterotoxigenic strains originated from cases of enteritis and included all three animal species. Apart from enterotoxigenicity, one C. perfringens strain produced beta toxin (type C) and 21 strains produced large amounts of alpha-toxin. The latter strains were predominantly associated with necrotic enteritis in chickens.
对从患有肠炎或其他疾病的牛、羊和鸡的肠道内容物中分离出的114株产气荚膜梭菌进行了产肠毒素能力的研究。采用了反向被动血凝试验、荧光抗体试验和免疫扩散试验。根据反向被动血凝试验的滴度,并得到其他两项试验的支持,将这些菌株的产肠毒素性任意分为两类:高产肠毒素性和潜在产肠毒素性,每类各占12%。所有高产肠毒素性菌株均来自肠炎病例,涉及所有三种动物。除了产肠毒素性外,一株产气荚膜梭菌产生β毒素(C型),21株产生大量α毒素。后一类菌株主要与鸡的坏死性肠炎有关。