实验性绵羊和犊牛对A型产气荚膜梭菌肠毒素的临床及抗体反应
Clinical and antibody responses to Clostridium perfringens type A enterotoxin in experimental sheep and calves.
作者信息
Niilo L, Cho H J
出版信息
Can J Comp Med. 1985 Apr;49(2):145-8.
Abstract
Clostridium perfringens type A live cultures or sonicated sporulating cells, all containing enterotoxin, were repeatedly inoculated into sheep and calves by the intraduodenal route over periods of 30 to 35 days. Serum antibody to C. perfringens enterotoxin, tested by ELISA, developed in four of seven sheep and in two of four calves. The titers ranged from 400 to 1600. The live organism introduced into the duodenum did not become established in the bacterial flora of the intestinal tract.
摘要
含有肠毒素的A型产气荚膜梭菌活培养物或经超声处理的产孢细胞，在30至35天的时间里，通过十二指肠内途径反复接种到绵羊和小牛体内。通过ELISA检测，7只绵羊中有4只、4只小牛中有2只产生了针对产气荚膜梭菌肠毒素的血清抗体。抗体效价范围为400至1600。引入十二指肠的活生物体未在肠道菌群中定植。
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