Niilo L
Can J Comp Med. 1979 Jan;43(1):98-101.
Three known enterotoxigenic Clostridium perfringens type A strains were mixed in various combinations with three nonenterotoxigenic strains and three lots of animal intestinal contents. They were grown as mixed cultures and tested for the presence of enterotoxin by the fluorescent antibody, reversed passive hemagglutination and immunodiffusion techniques. The fluorescent antibody and reversed passive hemagglutination tests detected enterotoxin in all 16 cultures prepared but the immunodiffusion test failed on two cultures. Attempts to reisolate the enterotoxigenic strains from the mixed cultures was successful in 12 cases when two of five isolated colonies were selected.
将三株已知的产肠毒素A型产气荚膜梭菌菌株与三株非产肠毒素菌株以及三批动物肠道内容物以各种组合混合。将它们作为混合培养物培养，并通过荧光抗体、反向被动血凝和免疫扩散技术检测肠毒素的存在。荧光抗体和反向被动血凝试验在制备的所有16种培养物中均检测到了肠毒素，但免疫扩散试验在两种培养物中未检测到。当从五个分离菌落中选择两个时，在12例中成功地从混合培养物中再次分离出产肠毒素菌株。
