Bio-Products Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research (CSIR), Adyar, Chennai, 600020, Tamilnadu, India.
Clin Chim Acta. 2011 Jan 30;412(3-4):386-9. doi: 10.1016/j.cca.2010.11.016. Epub 2010 Nov 18.
In human anatomy, a nail is a hornlike envelope covering the dorsal aspect of the terminal phalanges of fingers and toes. Nail disorders are most common among the geriatric population. Diabetes mellitus is also supposed to affect the condition of nails. Acceptable differences in infrared (IR) spectra of chronic and acute diabetes mellitus patient fingernail specimens compared to control normal specimens were investigated in this study.
Using a Nicolet 360 Fourier Transform Infra Red (FTIR) spectrometer, the spectra of the nails of diabetics and normal specimens were recorded.
In the case of non-diabetic patients, the amide I band was observed <1640 cm(-1) (1626, 1632, and 1638 cm(-1)). The bands around 1637 cm(-1), were attributable to amide I of β sheet structures. Amide II bands were absent in all the non-diabetic patients. Amide III bands around 1250 cm(-1) were observed both in diabetic and non-diabetic patients. In all the diabetic patients, a peak of <500 cm(-1), particularly around 468 cm(-1), was observed.
The proteins in the nails of diabetic patients contain α-helical structure, including the presence of amide II bonds. Alkyl thiolated structures are observed. Nails of non-diabetic patients do not have the amide II structures.
在人体解剖学中，指甲是一种覆盖手指和脚趾末端背侧的角蛋白套。指甲疾病在老年人群中最为常见。糖尿病也被认为会影响指甲的状况。本研究旨在研究慢性和急性糖尿病患者指甲标本与正常对照标本的红外（IR）光谱之间可接受的差异。
使用 Nicolet 360 傅里叶变换红外（FTIR）光谱仪记录指甲的光谱。
在非糖尿病患者中，酰胺 I 带观察到<1640 cm(-1)（1626、1632 和 1638 cm(-1)）。约 1637 cm(-1)处的带归因于β片层结构的酰胺 I。所有非糖尿病患者均不存在酰胺 II 带。在所有糖尿病患者中，观察到约 1250 cm(-1)处的酰胺 III 带，在糖尿病和非糖尿病患者中均存在。在所有糖尿病患者中，观察到<500 cm(-1)，特别是约 468 cm(-1)处的峰。
糖尿病患者指甲中的蛋白质含有α-螺旋结构，包括酰胺 II 键的存在。观察到烷基硫醇结构。非糖尿病患者的指甲没有酰胺 II 结构。
