人类胰岛分离的手术方面。
Surgical aspects of human islet isolation.
机构信息
Clinical Islet Laboratory and Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada.
出版信息
Islets. 2010 Sep-Oct;2(5):265-73. doi: 10.4161/isl.2.5.13019. Epub 2010 Sep 1.
Abstract
Islet transplantation is a safe and effective procedure; however, it depends on the critical step of isolating high quality of islets from whole pancreas. Human islet isolation requires considerable experience and expertise, and is frequently seen as 'an art rather than a science'. However, without scientific knowledge of pancreatic anatomy, real experience can not be gained. In particular, an understanding ductal anatomy is important to perform human islet isolation. This review is based on clinical experience of more than 900 human islet isolations performed over 10 years and aims to highlight pancreatic anatomy and surgical techniques in islet processing.
摘要
胰岛移植是一种安全有效的方法；然而，它取决于从整个胰腺中分离高质量胰岛的关键步骤。人胰岛的分离需要丰富的经验和专业知识，并且经常被视为“一门艺术而不是科学”。然而，如果没有对胰腺解剖学的科学认识，就无法获得真正的经验。特别是，理解导管解剖对于进行人胰岛分离非常重要。这篇综述基于 10 多年来进行的超过 900 次人胰岛分离的临床经验，旨在强调胰岛处理中的胰腺解剖和手术技术。
相似文献
1
Surgical aspects of human islet isolation.人类胰岛分离的手术方面。
Islets. 2010 Sep-Oct;2(5):265-73. doi: 10.4161/isl.2.5.13019. Epub 2010 Sep 1.
2
Heterogeneity of human pancreata in perspective of the isolation of the islets of langerhans.从胰岛分离角度看人类胰腺的异质性。
Transplant Proc. 2008 Mar;40(2):367-9. doi: 10.1016/j.transproceed.2007.12.022.
3
Efficacy of human islet isolation from the tail section of the pancreas for the possibility of living donor islet transplantation.从胰腺尾部进行人胰岛分离用于活体供体胰岛移植可能性的疗效。
Transplantation. 2004 Sep 27;78(6):839-43. doi: 10.1097/01.tp.0000130177.03326.d5.
4
Calcium: A Crucial Potentiator for Efficient Enzyme Digestion of the Human Pancreas.钙：高效酶解人胰腺的关键增强剂。
Cell Transplant. 2018 Jul;27(7):1031-1038. doi: 10.1177/0963689718779350. Epub 2018 Jun 26.
5
Seven consecutive successful clinical islet isolations with pancreatic ductal injection.七例连续成功的经胰管内注射胰腺胰岛分离术。
Cell Transplant. 2010;19(3):291-7. doi: 10.3727/096368909X481773. Epub 2009 Dec 12.
6
Pancreas anatomy and surgical procedure for pancreatectomy in rhesus monkeys.恒河猴胰腺解剖及胰腺切除术的手术操作
J Med Primatol. 2011 Dec;40(6):376-82. doi: 10.1111/j.1600-0684.2011.00499.x. Epub 2011 Sep 6.
7
Analysis on donor and isolation-related factors of successful isolation of human islet of Langerhans from human cadaveric donors.人类尸体供体来源的胰岛成功分离的供体及分离相关因素分析
Transplant Proc. 2005 Oct;37(8):3402-3. doi: 10.1016/j.transproceed.2005.09.055.
8
Technical aspects involved in the harvesting and preservation of the pancreas used for pancreatic islet allotransplantation.用于胰岛同种异体移植的胰腺获取与保存所涉及的技术方面。
Chirurgia (Bucur). 2013 May-Jun;108(3):372-80.
9
Heterogeneity of Human Pancreatic Islet Isolation Around Europe: Results of a Survey Study.欧洲人类胰腺胰岛分离的异质性：一项调查研究的结果。
Transplantation. 2020 Jan;104(1):190-196. doi: 10.1097/TP.0000000000002777.
10
Pancreatic ductal perfusion at organ procurement enhances islet yield in human islet isolation.器官获取时的胰管灌注可提高人胰岛分离中的胰岛产量。
Pancreas. 2014 Nov;43(8):1249-1255. doi: 10.1097/MPA.0000000000000196.
引用本文的文献
1
Sequential islet transplants for type 1 diabetes are not associated with sustained or cumulative increases in hepatic portal venous pressure.1型糖尿病的序贯胰岛移植与肝门静脉压力的持续或累积升高无关。
PLoS One. 2025 Jul 29;20(7):e0329074. doi: 10.1371/journal.pone.0329074. eCollection 2025.
2
Molecular correlates of glycine receptor activity in human β cells.人β细胞中甘氨酸受体活性的分子关联
Mol Metab. 2025 Jun;96:102156. doi: 10.1016/j.molmet.2025.102156. Epub 2025 Apr 19.
3
The effects of free fatty acid-free bovine serum albumin and palmitate on pancreatic β-cell function.无游离脂肪酸的牛血清白蛋白和棕榈酸酯对胰腺β细胞功能的影响。
Islets. 2025 Dec;17(1):2479911. doi: 10.1080/19382014.2025.2479911. Epub 2025 Mar 16.
4
Human research islet cell culture outcomes at the Alberta Diabetes Institute IsletCore.艾伯塔糖尿病研究所胰岛核心的人类胰岛细胞培养结果。
Islets. 2024 Dec 31;16(1):2385510. doi: 10.1080/19382014.2024.2385510. Epub 2024 Aug 4.
5
HumanIslets: An integrated platform for human islet data access and analysis.人类胰岛：一个用于人类胰岛数据访问和分析的集成平台。
bioRxiv. 2024 Jun 22:2024.06.19.599613. doi: 10.1101/2024.06.19.599613.
6
beta-cell killing models using immune cells and human pluripotent stem cell-derived islets: Challenges and opportunities.使用免疫细胞和人多能干细胞衍生胰岛的β细胞杀伤模型：挑战与机遇。
Front Endocrinol (Lausanne). 2023 Jan 16;13:1076683. doi: 10.3389/fendo.2022.1076683. eCollection 2022.
7
Ca2+ Oscillations, Waves, and Networks in Islets From Human Donors With and Without Type 2 Diabetes.胰岛中源自 2 型糖尿病患者和非糖尿病患者的钙离子震荡、波和网络
Diabetes. 2022 Dec 1;71(12):2584-2596. doi: 10.2337/db22-0004.
8
Mesenchymal Stem Cells Secretions Enhanced ATP Generation on Isolated Islets during Transplantation.间质干细胞分泌液增强移植过程中分离胰岛的 ATP 生成。
Islets. 2022 Dec 31;14(1):69-81. doi: 10.1080/19382014.2021.2022423.
9
Improved glucose tolerance with DPPIV inhibition requires β-cell SENP1 amplification of glucose-stimulated insulin secretion.DPPIV 抑制作用改善葡萄糖耐量需要 β 细胞 SENP1 扩增葡萄糖刺激的胰岛素分泌。
Physiol Rep. 2020 Apr;8(8):e14420. doi: 10.14814/phy2.14420.
10
Systematic review of islet cryopreservation.胰岛冷冻保存的系统评价
Islets. 2018 Jan 2;10(1):40-49. doi: 10.1080/19382014.2017.1405202. Epub 2018 Jan 9.
本文引用的文献
1
Detection of Microbial Contamination during Human Islet Isolation.人胰岛分离过程中微生物污染的检测
Cell Transplant. 2007 Jan;16(1):9-13. doi: 10.3727/000000007783464498.
2
Partial dorsal agenesis accompanied with circumportal pancreas in a donor for islet transplantation.胰岛移植供体中伴有门周胰腺的部分背侧发育不全。
Islets. 2010 May-Jun;2(3):146-8. doi: 10.4161/isl.2.3.11715.
3
Histologic graft assessment after clinical islet transplantation.临床胰岛移植后的组织学移植物评估。
Transplantation. 2009 Dec 15;88(11):1286-93. doi: 10.1097/TP.0b013e3181bc06b0.
4
Donor-derived malignancy in a pancreas graft.胰腺移植受者发生供体来源的恶性肿瘤。
Transpl Int. 2010 May 1;23(5):e5-6. doi: 10.1111/j.1432-2277.2009.00989.x. Epub 2009 Nov 5.
5
Portal annular pancreas, a notable pancreatic malformation: frequency, morphology, and implications for pancreatic surgery.门静脉环周胰腺，一种显著的胰腺畸形：发生率、形态及对胰腺手术的影响
Surgery. 2009 Sep;146(3):515-8. doi: 10.1016/j.surg.2009.03.018. Epub 2009 May 9.
6
Circumportal pancreas with retroportal main pancreatic duct.门静脉周围胰腺伴门静脉后主胰管。
Pancreas. 2009 Aug;38(6):713-5. doi: 10.1097/MPA.0b013e3181a910ca.
7
Circumportal pancreas and islet isolation.门静脉周围胰腺及胰岛分离。
Surgery. 2009 Jul;146(1):126-7. doi: 10.1016/j.surg.2008.04.017. Epub 2008 Aug 10.
8
Resection of the intrapancreatic bile duct preserving the pancreas.保留胰腺的胰内胆管切除术。
J Hepatobiliary Pancreat Surg. 2009;16(1):31-4. doi: 10.1007/s00534-008-0013-2. Epub 2008 Dec 17.
9
Pig pancreas anatomy: implications for pancreas procurement, preservation, and islet isolation.猪胰腺解剖结构：对胰腺获取、保存及胰岛分离的影响
Transplantation. 2008 Dec 15;86(11):1503-10. doi: 10.1097/TP.0b013e31818bfda1.
10
Neonatal and late-onset diabetes mellitus caused by failure of pancreatic development: report of 4 more cases and a review of the literature.胰腺发育不全所致新生儿及晚发型糖尿病：4例报告及文献复习
Pediatrics. 2008 Jun;121(6):e1541-7. doi: 10.1542/peds.2007-3543.
Zotero 插件