人轮状病毒对细胞培养物中抗原掺入及感染性的增强作用
Enhancement of antigen incorporation and infectivity of cell cultures by human rotavirus.
作者信息
Schoub B D, Kalica A R, Greenberg H B, Bertran D M, Sereno M M, Wyatt R G, Chanock R M, Kapikian A Z
出版信息
J Clin Microbiol. 1979 Apr;9(4):488-92. doi: 10.1128/jcm.9.4.488-492.1979.
Infection of cell cultures with human rotavirus preparations was attempted and the effects of trypsin and low-speed centrifugation on antigen incorporation, as demonstrated by immunofluorescence and radioimmunoassay, were determined. In addition, the effect of viral aggregation on antigen incorporation was investigated by filtering viral preparations. Four strains of human rotavirus were employed, and the results were compared to those obtained with two tissue culture-adapted animal rotaviruses. Centrifugation and trypsin appeared to have little or no effect on infectivity of the tissue culture-adapted (simian rotavirus) or -adaptable (Nebraska calf diarrhea virus) strains, whereas centrifugation and viral aggregation appeared to be essential for the human viruses. In addition, trypsin enhanced antigen incorporation of the human strains to some extent. Infectivity for cell cultures and in vitro human rotavirus protein formation was demonstrated by [35S]methionine incorporation, and the specificity of this human viral protein was established by radio-immunoprecipitation.
尝试用人轮状病毒制剂感染细胞培养物，并通过免疫荧光和放射免疫测定法确定胰蛋白酶和低速离心对抗原掺入的影响。此外，通过过滤病毒制剂研究病毒聚集对抗原掺入的影响。使用了四株人轮状病毒，并将结果与用两株适应组织培养的动物轮状病毒获得的结果进行比较。离心和胰蛋白酶似乎对适应组织培养的（猿猴轮状病毒）或可适应的（内布拉斯加小牛腹泻病毒）毒株的感染性影响很小或没有影响，而离心和病毒聚集似乎对人病毒至关重要。此外，胰蛋白酶在一定程度上增强了人毒株的抗原掺入。通过[35S]甲硫氨酸掺入证明了对细胞培养物的感染性和体外人轮状病毒蛋白的形成，并通过放射免疫沉淀确定了这种人病毒蛋白的特异性。
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