不同阶段 COPD 中 I 型和 III 型胶原前体蛋白的变异性。
Variability in the precursor proteins of collagen I and III in different stages of COPD.
机构信息
Institute of Clinical Medicine, Department of Internal Medicine, Respiratory Unit, Centre of Excellence in Research, P, O, Box 5000, 90014 University of Oulu, Oulu, Finland.
出版信息
Respir Res. 2010 Nov 30;11(1):165. doi: 10.1186/1465-9921-11-165.
BACKGROUND
Levels of precursor proteins of collagen I and III are increased in fibrotic pulmonary diseases. This study determined whether the expression of precursors of type I and III collagen proteins would be increased in small and large airways of COPD patients in various stages of the disease reflecting fibrogenesis.
METHODS
The levels of precursor proteins of collagen I and III were studied by immunohistochemistry and quantified by image analysis in lung tissue of 16 non-smokers, 20 smokers with normal lung function, 20 smokers with stage I-II COPD and 8 ex-smokers with stage IV COPD.
RESULTS
In large airways, the subepithelial layer which was positive for precursor proteins of collagen I and III was thicker in smokers and in stage I-II COPD compared to non-smokers. Large airways in stage IV COPD showed reduced expression of precursor protein of collagen I whereas precursor of collagen III was increased. The amount of precursor protein of collagen III was increased in small airways of smokers and stage I-II COPD but reduced in stage IV COPD.
CONCLUSIONS
Precursor proteins of collagen I and III revealed different expression profiles in large and small airways in various stages of COPD. Smoking enhanced expression of both precursors in large airways with a positive correlation with pack-years.
背景
I 型和 III 型胶原前体蛋白的水平在纤维性肺疾病中增加。本研究旨在确定在 COPD 患者的大小气道中，I 型和 III 型胶原前体蛋白的表达是否会随着疾病的不同阶段而增加，从而反映纤维化的发生。
方法
通过免疫组织化学方法研究了胶原 I 和 III 前体蛋白的水平，并通过图像分析对 16 名不吸烟者、20 名肺功能正常的吸烟者、20 名 I 期- II 期 COPD 患者和 8 名 IV 期 COPD 戒烟者的肺组织中的前体蛋白进行了定量分析。
结果
在大气道中，I 型和 III 型胶原前体蛋白阳性的上皮下层在吸烟者和 I 期- II 期 COPD 患者中比不吸烟者更厚。IV 期 COPD 的大气道中 I 型胶原前体蛋白的表达减少，而 III 型胶原前体蛋白增加。吸烟者和 I 期- II 期 COPD 的小气道中 III 型胶原前体蛋白的含量增加，但在 IV 期 COPD 中减少。
结论
在 COPD 的不同阶段，I 型和 III 型胶原前体蛋白在大气道和小气道中表现出不同的表达谱。吸烟增强了大气道中两种前体蛋白的表达，与吸烟包年数呈正相关。
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