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单锥形纳米孔内附着的 DNA 链:离子孔特性和对 DNA 生物物理学的深入了解。

DNA strands attached inside single conical nanopores: ionic pore characteristics and insight into DNA biophysics.

机构信息

Department of Physics and Astronomy, University of California, Irvine, CA 92697, USA.

出版信息

J Membr Biol. 2011 Jan;239(1-2):105-13. doi: 10.1007/s00232-010-9328-4. Epub 2010 Dec 1.

Abstract

Single nanopores attract a great deal of scientific interest as a basis for biosensors and as a system to study the interactions and behavior of molecules in a confined volume. Tuning the geometry and surface chemistry of nanopores helps create devices that control transport of ions and molecules in solution. Here, we present single conically shaped nanopores whose narrow opening of 8 or 12 nm is modified with single-stranded DNA molecules. We find that the DNA occludes the narrow opening of nanopores and that the blockade extent decreases with the ionic strength of the background electrolyte. The results are explained by the ionic strength dependence of the persistence length of DNA. At low KCl concentrations (10 mM) the molecules assume an extended and rigid conformation, thereby blocking the pore lumen and reducing the flow of ionic current to a greater extent than compacted DNA at high salt concentrations. Attaching flexible polymers to the pore walls hence creates a system with tunable opening diameters in order to regulate transport of both neutral and charged species.

摘要

单纳米孔作为生物传感器的基础和研究分子在受限体积中相互作用和行为的系统引起了极大的科学兴趣。调整纳米孔的几何形状和表面化学性质有助于创建控制溶液中离子和分子输运的器件。在这里,我们提出了具有单锥形纳米孔的单纳米孔,其 8 或 12nm 的狭窄开口用单链 DNA 分子修饰。我们发现 DNA 阻塞纳米孔的狭窄开口,并且阻塞程度随背景电解质的离子强度而降低。结果通过 DNA 持久长度对离子强度的依赖性来解释。在低 KCl 浓度(10mM)下,分子呈现伸展和刚性构象,从而比在高盐浓度下更紧密的 DNA 更大程度地阻塞孔腔并减少离子电流的流动。将柔性聚合物附着到孔壁上,从而创建了具有可调节开口直径的系统,以调节中性和带电物质的传输。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9929/3030949/c0e4c824d9ac/232_2010_9328_Fig1_HTML.jpg

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