Chen Peng, Gu Jiajun, Brandin Eric, Kim Young-Rok, Wang Qiao, Branton Daniel
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Division of Engineering & Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
Nano Lett. 2004 Nov;4(11):2293-2298. doi: 10.1021/nl048654j.
Nanopores can serve as high throughput, single molecule sensing devices that provide insight into the distribution of static and dynamic molecular activities, properties, or interactions. We have studied double stranded DNA electrophoretic transport dynamics through fabricated nanopores in silicon nitride. A fabricated pore enables us to interrogate a broader range of molecules with a wider range of conditions than can be investigated in a self-assembled protein pore in a lipid membrane.
纳米孔可作为高通量单分子传感装置,用于深入了解静态和动态分子活性、特性或相互作用的分布情况。我们研究了双链DNA在氮化硅中制造的纳米孔中的电泳传输动力学。与脂质膜中自组装蛋白孔相比,制造的孔使我们能够在更广泛的条件下研究更广泛的分子。