Rothman J H, Raymond C K, Gilbert T, O'Hara P J, Stevens T H
Institute of Molecular Biology, University of Oregon, Eugene 97403.
Cell. 1990 Jun 15;61(6):1063-74. doi: 10.1016/0092-8674(90)90070-u.
Members of the Mx protein family promote interferon-inducible resistance to viral infection in mammals and act by unknown mechanisms. We identified an Mx-like protein in yeast and present genetic evidence for its cellular function. This protein, the VPS1 product, is essential for vacuolar protein sorting, normal organization of intracellular membranes, and growth at high temperature, implying that Mx-like proteins are engaged in fundamental cellular processes in eukaryotes. Vps1p contains a tripartite GTP binding motif, which suggests that binding to GTP is essential to its role in protein sorting. Vps1p-specific antibody labels punctate cytoplasmic structures that condense to larger structures in a Golgi-accumulating sec7 mutant; thus, Vps1p may associate with an intermediate organelle of the secretory pathway.
Mx蛋白家族成员可促进哺乳动物中干扰素诱导的抗病毒感染能力，其作用机制尚不清楚。我们在酵母中鉴定出一种类似Mx的蛋白，并提供了其细胞功能的遗传学证据。这种蛋白即VPS1产物，对于液泡蛋白分选、细胞内膜的正常组织以及在高温下的生长至关重要，这意味着类似Mx的蛋白参与了真核生物的基本细胞过程。Vps1p包含一个三联体GTP结合基序，这表明与GTP的结合对其在蛋白分选中的作用至关重要。Vps1p特异性抗体标记点状细胞质结构，这些结构在高尔基体积累的sec7突变体中凝聚成更大的结构；因此，Vps1p可能与分泌途径的中间细胞器相关联。
