Department of Orthopedics Surgery, The Second Affiliated Hospital of Medical College, Zhejiang University, Jie Fang Road 88#, 310009 Hangzhou, People's Republic of China.
Exp Biol Med (Maywood). 2010 Dec;235(12):1425-31. doi: 10.1258/ebm.2010.010174.
Thymoquinone (TQ) is the main constituent of Nigella sativa oil, which has been traditionally used against arthritis in the Middle East. In this study, we investigated the effect of TQ against matrix metalloproteinase (MMP) expression in both rabbit chondrocytes and animal mode of osteoarthritis (OA) induced by anterior cruciate ligament transection and tested whether or not nuclear factor kappa B (NF-κB) was involved in this process. TQ down-regulated MMP-1, MMP-3 and MMP-13 expression and up-regulated tissue inhibitors of metalloproteinase-1 expression as assessed by quantitative realtime polymerase chain reaction. In addition, NF-κB p65 protein level as well as its translocation induced by interleukin-1β were inhibited by TQ. Our findings suggest the potential of TQ in the treatment of OA.
姜酮（TQ）是黑种草籽油的主要成分，传统上被用于治疗中东地区的关节炎。在这项研究中，我们研究了 TQ 对兔软骨细胞基质金属蛋白酶（MMP）表达的影响，以及 TQ 对前交叉韧带切断术诱导的动物骨关节炎（OA）模型的影响，并检测核因子 kappa B（NF-κB）是否参与了这一过程。TQ 通过定量实时聚合酶链反应下调 MMP-1、MMP-3 和 MMP-13 的表达，并上调组织金属蛋白酶抑制剂-1 的表达。此外，TQ 还抑制了白细胞介素-1β诱导的 NF-κB p65 蛋白水平及其易位。我们的研究结果表明 TQ 在治疗 OA 方面具有潜力。
