Division of Plant Biology, Bose Institute, Kolkata, WB, India.
J Proteomics. 2011 Mar 1;74(3):337-49. doi: 10.1016/j.jprot.2010.11.012. Epub 2010 Dec 3.
The role of salicylic acid (SA) in inducing resistance to MYMIV infection in Vigna mungo has been elucidated by proteomics. Twenty-nine proteins identified by MALDI-TOF/TOF, predicted to be involved in stress responses, metabolism, photosynthesis, transport and signal transduction, showed increased abundance upon SA treatment. Susceptible plants showed characteristic yellow mosaic symptoms upon MYMIV infection. A concentration dependent decrease in physiological symptoms associated with MYMIV was observed upon exogenous SA treatment prior to viral inoculation; and no visible symptom was observed at 100 μM SA. SA treatment stimulated SOD and GPX activity and inhibited CAT activity thus preventing ROS mediated damage. Significant increase in chlorophyll, protein, carbohydrate, phenolic content and H(2)O(2) were observed. Involvement of calmodulin for transmission of defense signal by SA is suggested. A metabolic reprogramming leading to enhanced synthesis of proteins involved in primary and secondary metabolisms is necessary for SA mediated resistance to MYMIV. Identification of proteins showing increased abundance, involved in photosynthetic process is a significant finding which restores virus-induced degradation of the photosynthetic apparatus and provides enhanced metabolites required for repartition of resources towards defense.
水杨酸（SA）在豇豆对 MYMIV 感染诱导抗性中的作用通过蛋白质组学得到了解析。通过 MALDI-TOF/TOF 鉴定的 29 种蛋白质，预测其参与应激反应、代谢、光合作用、运输和信号转导，在 SA 处理后丰度增加。易感植物在 MYMIV 感染后表现出典型的黄色花叶症状。在病毒接种前用外源 SA 处理观察到与 MYMIV 相关的生理症状呈浓度依赖性下降；在 100 μM SA 下未观察到可见症状。SA 处理刺激 SOD 和 GPX 活性，抑制 CAT 活性，从而防止 ROS 介导的损伤。观察到叶绿素、蛋白质、碳水化合物、酚类含量和 H₂O₂的显著增加。提示 SA 通过钙调蛋白传递防御信号。SA 介导的对 MYMIV 的抗性需要代谢重编程，从而增强参与初级和次级代谢的蛋白质的合成。鉴定出丰度增加的、参与光合作用过程的蛋白质是一个重要发现，它恢复了病毒诱导的光合装置的降解，并提供了增强的代谢物，以重新分配资源用于防御。
