Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Søltofts Plads 221, 2800 Kgs, Lyngby, Denmark.
J Ind Microbiol Biotechnol. 2011 Sep;38(9):1329-35. doi: 10.1007/s10295-010-0916-1. Epub 2010 Dec 4.
Tolypocladium inflatum is known primarily for its production of the cyclosporines that are used as an immunosuppressive drug. However, we report here the production of the carcinogenic fumonisins B(2) and B(4) by this biotechnologically relevant fungal genus. These mycotoxins were detected in 11 strains tested from three species: Tolypocladium inflatum, T. cylindrosporum, and T. geodes. Production of fumonisins by Fusarium spp. and Aspergillus niger is highly medium- and temperature-dependent, so the effect of these parameters on fumonisin production by three T. inflatum strains was studied. Maximum production was achieved on media with high sugar content incubated at 25-30°C. Since these results demonstrate that fumonisin production could be widespread within the genus Tolypocladium, the potential contamination of commercial cyclosporine preparations with fumonisins needs to be investigated.
膨状被孢霉主要因其能产生环孢菌素而闻名，环孢菌素可用作免疫抑制剂。然而，我们在此报告称，这种在生物技术上具有重要意义的真菌属也能产生致癌的伏马菌素 B(2)和 B(4)。在所测试的三个物种（膨状被孢霉、柱孢被孢霉和地衣被孢霉）的 11 株菌中均检测到了这些霉菌毒素。伏马菌素 B(2)和 B(4)由镰刀菌属和黑曲霉产生，其产量高度依赖于培养基和温度，因此研究了这三个膨状被孢霉菌株在不同参数条件下的伏马菌素产量。在 25-30°C 温度下，高糖含量培养基中可实现最大产量。由于这些结果表明，伏马菌素的产生可能在膨状被孢霉属中广泛存在，因此需要对商业环孢菌素制剂可能受到伏马菌素污染的情况进行调查。
