Engineering Research Center in Biomaterials, Sichuan University, 610064, Chengdu, China.
J Mater Sci Mater Med. 2011 Feb;22(2):371-9. doi: 10.1007/s10856-010-4198-y. Epub 2010 Dec 8.
In order to enhance the ability of calcium phosphate-based biomaterials for bone defect repair, icariin (Ica), one natural product with ability of promoting osteoblasts differentiation in vitro and enhancing bone formation in vivo, was loaded into porous β-tricalcium phosphate ceramic (β-TCP) disks. The obtained Ica-loaded porous β-TCP ceramic (Ica/β-TCP) disks were characterized by SEM. The SEM photos indicated that the disks had porous structure and the surface morphology of the porous β-TCP ceramic (β-PTCP) disks had no obvious difference from the Ica/β-TCP disks. The Ica release curve of Ica/β-TCP disks showed a burst release during the first 1 day and the concentration of released Ica during the first 3 days had low cytotoxicity. The loading Ica in Ica/β-TCP disks hardly affected the attachment and morphology of Ros17/28 cells, however, the Ica/β-TCP disks were favorable to supporting the proliferation and differentiation of Ros17/28 cells better compared with the β-PTCP disks. There was plenty of bone-like apatite formed on the surface of Ica/β-TCP disks soaked in SBF solution for three days. After back intramuscular implantation of rats for three months, no obvious osteogenic evidence was detected in β-PTCP disks, but new bone formation was observed in Ica/β-TCP disks. Fibrous tissues and slight inflammatory reaction was also found in the Ica/β-TCP disks and β-TCP disks. Therefore, the loading Ica did not change the biocompatibility of β-TCP ceramic, but enhanced the bioactivity of β-TCP ceramic in vivo. The Ica/β-TCP ceramic had potential to be used for bone defect repair.
为了提高磷酸钙基生物材料修复骨缺损的能力，将淫羊藿苷（Ica）——一种具有体外促进成骨细胞分化和体内增强骨形成能力的天然产物——载入多孔β-磷酸三钙陶瓷（β-TCP）盘中。用 SEM 对负载 Ica 的多孔β-TCP 陶瓷（Ica/β-TCP）盘进行了表征。SEM 照片表明，这些盘具有多孔结构，多孔β-TCP 陶瓷（β-PTCP）盘的表面形态与 Ica/β-TCP 盘没有明显区别。Ica/β-TCP 盘的 Ica 释放曲线在第 1 天表现出突释，前 3 天释放的 Ica 浓度具有低细胞毒性。Ica/β-TCP 盘中负载的 Ica 几乎不影响 Ros17/28 细胞的附着和形态，但与β-PTCP 盘相比，Ica/β-TCP 盘更有利于支持 Ros17/28 细胞的增殖和分化。在 SBF 溶液中浸泡 3 天后，Ica/β-TCP 盘表面形成了大量类骨质磷灰石。在大鼠肌肉内回植 3 个月后，β-PTCP 盘中未见明显成骨证据，但在 Ica/β-TCP 盘中观察到新骨形成。在 Ica/β-TCP 盘和β-TCP 盘中也发现了纤维组织和轻微的炎症反应。因此，负载 Ica 并没有改变β-TCP 陶瓷的生物相容性，但增强了β-TCP 陶瓷在体内的生物活性。Ica/β-TCP 陶瓷具有用于骨缺损修复的潜力。
