Choi Somang, Noh Sung Hyun, Lim Chae Ouk, Kim Hak-Jun, Jo Han-Saem, Min Ji Seon, Park Kyeongsoon, Kim Sung Eun
Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 08308, Korea.
Department of Neurosurgery, National Health Insurance Service Ilsan Hospital, #100, Ilsan-ro, Ilsan-donggu, Goyang-si, Gyeonggi-do 10444, Korea.
Nanomaterials (Basel). 2020 Oct 20;10(10):2071. doi: 10.3390/nano10102071.
Nanodiamonds (NDs) have been used as drug delivery vehicles due to their low toxicity and biocompatibility. Recently, it has been reported that NDs have also osteogenic differentiation capacity. However, their capacity using NDs alone is not enough. To significantly improve their osteogenic activity, we developed icariin (ICA)-functionalized NDs (ICA-NDs) and evaluated whether ICA-NDs enhance their in vitro osteogenic capacity. Unmodified NDs and ICA-NDs showed nanosized particles that were spherical in shape. The ICA-NDs achieved a prolonged ICA release for up to 4 weeks. The osteogenic capacities of NDs, ICA (10 μg)-NDs, and ICA (50 μg)-NDs were demonstrated by alkaline phosphatase (ALP) activity; calcium content; and mRNA gene levels of osteogenic-related markers, including ALP, runt-related transcript factor 2 (RUNX2), collagen type I alpha 1 (COL1A1), and osteopontin (OPN). In vitro cell studies revealed that ICA (50 μg)-ND-treated MC3T3-E1 cells greatly increased osteogenic markers, including ALP, calcium content, and mRNA gene levels of osteogenic-related markers, including ALP, RUNX2, COL1A1, and OPN compared to ICA (10 μg)-NDs or ND-treated cells. These our data suggest that ICA-NDs can promote osteogenic capacity.
纳米金刚石（NDs）因其低毒性和生物相容性而被用作药物递送载体。最近，有报道称NDs也具有成骨分化能力。然而，仅使用NDs的能力是不够的。为了显著提高其成骨活性，我们开发了淫羊藿苷（ICA）功能化的NDs（ICA-NDs），并评估了ICA-NDs是否能增强其体外成骨能力。未修饰的NDs和ICA-NDs呈现出球形的纳米颗粒。ICA-NDs实现了长达4周的ICA持续释放。通过碱性磷酸酶（ALP）活性、钙含量以及成骨相关标志物（包括ALP、 runt相关转录因子2（RUNX2）、I型胶原α1（COL1A1）和骨桥蛋白（OPN））的mRNA基因水平来证明NDs、ICA（10μg）-NDs和ICA（50μg）-NDs的成骨能力。体外细胞研究表明，与ICA（10μg）-NDs或ND处理的细胞相比，ICA（50μg）-ND处理的MC3T3-E1细胞显著增加了成骨标志物，包括ALP、钙含量以及成骨相关标志物（包括ALP、RUNX2、COL1A1和OPN）的mRNA基因水平。我们的数据表明ICA-NDs可以促进成骨能力。
