JST domestic fellow, Tohoku Research Center of Forestry and Forest Products Research Institute, Nabeyashiki 92-25, Shimo-Kuriyagawa, Morioka, Iwate 020-0123, Japan.
Mycologia. 2004 May-Jun;96(3):548-57.
Leptographium species are anamorphs of Ophiostoma, commonly isolated from conifer. There are, however, a small number of these fungi that have been collected from angiosperm hosts. In this study, we describe Leptographium pruni, sp. nov. isolated from the bark of Prunus jamasakura infested by the bark beetle Polygraphus ssiori. This new species is unusual in having a distinct Sporothrix synanamorph with ramoconidia. No evidence of a teleomorph was found, but a high level of tolerance to the antibiotic cycloheximide and the presence of a Sporothrix synanamorph suggest that L. pruni is an Ophiostoma anamorph. Analysis of sequence data for the domain 1 region of the LSUrDNA operon also supports the phylogenetic relationship of L. pruni with Ophiostoma. In addition, sequence data suggest that L. pruni is related to other species of Leptographium rather than Pesotum species with distinct Sporothrix synanamorphs.
节孔菌属真菌是小穴壳属的无性型，通常从针叶树中分离出来。然而，也有一小部分真菌是从被子植物宿主中收集到的。在这项研究中，我们描述了从被树皮甲虫 Polygraphus ssiori 侵害的樱桃李树皮中分离出来的新种李叶孔菌 Leptographium pruni，sp. nov.。这个新种的特点是具有明显的帚状枝分生孢子的 Sporothrix synanamorph。没有发现有性型的证据，但对环丝氨酸抗生素的高度耐受性和 Sporothrix synanamorph 的存在表明，L. pruni 是小穴壳属的无性型。 LSUrDNA 操纵子域 1 区序列数据分析也支持 L. pruni 与小穴壳属的系统发育关系。此外，序列数据表明，L. pruni 与其他节孔菌属的种有关，而不是与具有明显帚状枝分生孢子的 Pesotum 种有关。
