Torres J, Jennische E, Lange S, Lönnroth I
Department of Medical Microbiology, Gothenburg University, Sweden.
Gut. 1990 Jul;31(7):781-5. doi: 10.1136/gut.31.7.781.
The action of toxins A, B, and C from Clostridium difficile was studied in the small intestine and colon of rats. All three caused fluid accumulation in the small intestine, maximal secretion being induced by 1 micrograms of toxin A, 20 micrograms of B, and 15 micrograms of C. Both toxins A and C caused shedding of epithelial cells from the villi without visible damage to crypt cells; toxin A caused further extensive necrosis and bleeding. Toxin B caused secretion without visible damage to the epithelial cells, though this activity was unstable and decreased significantly after one week of storage. In the colon, toxin A caused secretion and shedding of surface epithelial cells without damage to crypt cells, toxin C caused only a weak secretion, and toxin B had no effect at all. In terms of immunohistochemistry, it was found that toxin A bound to the enterocytes at the tips of the villi but not to goblet and crypt cells. The complex expression or interaction of the toxins produced by Cl difficile may explain the broad spectrum of disease (diarrhoea, colitis, and pseudomembranous colitis) associated with this micro-organism.
研究了艰难梭菌毒素A、B和C在大鼠小肠和结肠中的作用。这三种毒素均可导致小肠内液体蓄积，1微克毒素A、20微克毒素B和15微克毒素C可诱导最大分泌量。毒素A和C均可导致绒毛上皮细胞脱落，但隐窝细胞未见明显损伤；毒素A可导致进一步广泛坏死和出血。毒素B可引起分泌，但对上皮细胞未见明显损伤，不过这种活性不稳定，储存一周后显著降低。在结肠中，毒素A可引起表面上皮细胞分泌和脱落，但对隐窝细胞无损伤，毒素C仅引起微弱分泌，毒素B则完全无作用。在免疫组织化学方面，发现毒素A与绒毛尖端的肠上皮细胞结合，但不与杯状细胞和隐窝细胞结合。艰难梭菌产生的毒素的复杂表达或相互作用可能解释了与这种微生物相关的广泛疾病谱（腹泻、结肠炎和假膜性结肠炎）。
