用于图案化细胞膜切割的光热纳米刀片

Wu Ting-Hsiang, Teslaa Tara, Teitell Michael A, Chiou Pei-Yu

Department of Electrical Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.

Opt Express. 2010 Oct 25;18(22):23153-60. doi: 10.1364/OE.18.023153.

We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized and specifically shaped explosive vapor bubble. Rapid bubble expansion and collapse punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The membrane cutting pattern is controlled by the metallic nanostructure configuration, laser pulse polarization, and energy. Highly controllable, sub-micron sized circular hole pairs to half moon-like, or cat-door shaped, membrane cuts were realized in glutaraldehyde treated HeLa cells.

我们报道了一种光热纳米刀片，它利用金属纳米结构收集短激光脉冲能量，并将其转化为高度局部化且形状特定的爆炸蒸汽泡。快速的气泡膨胀和坍塌通过高速流体流动和诱导的瞬态剪切应力刺穿轻轻接触的细胞膜。膜切割模式由金属纳米结构配置、激光脉冲偏振和能量控制。在戊二醛处理的HeLa细胞中实现了高度可控的亚微米尺寸圆孔对到半月形或猫门形的膜切割。