Program in Neuroscience, Department of Biological Science, Florida State University, Tallahassee, Florida 32306-4295, USA.
Learn Mem. 2010 Dec 17;18(1):19-23. doi: 10.1101/lm.2026311. Print 2011 Jan.
We investigated the involvement of PKA and PKC signaling in a negatively reinforced operant learning paradigm in Aplysia, learning that food is inedible (LFI). In vivo injection of PKA or PKC inhibitors blocked long-term LFI memory formation. Moreover, a persistent phase of PKA activity, although not PKC activity, was necessary for long-term memory. Surprisingly, neither PKA nor PKC activity was required for associative short-term LFI memory. Additionally, PKA and PKC were not required for the retrieval of short- or long-term memory (STM and LTM, respectively). These studies have identified key differences between the mechanisms underlying nonassociative sensitization, operant reward learning, and LFI memory in Aplysia.
我们研究了蛋白激酶 A（PKA）和蛋白激酶 C（PKC）信号在海兔负强化操作性学习范式中的作用，该范式学习到食物是不可食用的（LFI）。体内注射 PKA 或 PKC 抑制剂可阻断长期 LFI 记忆的形成。此外，PKA 活性的持续阶段，尽管不是 PKC 活性，对于长期记忆是必需的。令人惊讶的是，PKA 和 PKC 活性都不是联想性短期 LFI 记忆所必需的。此外，PKA 和 PKC 都不参与短期或长期记忆（STM 和 LTM，分别）的检索。这些研究确定了非联想性敏感化、操作性奖励学习和 Aplysia 中 LFI 记忆的机制之间的关键差异。
