Vihko V, Salminen A, Rantamäki J
Acta Physiol Scand. 1978 Sep;104(1):74-81. doi: 10.1111/j.1748-1716.1978.tb06253.x.
The activity of certain enzymes of energy metabolism (cytochrome c oxidase, citrate synthase, malate dehydrogenase, and lactate dehydrogenase) and of lysosomes (beta-glucuronidase, beta-N-acetylglucosamindase, arylsuphatase, ribonuclease, deoxyribonuclease, acid phosphatase, and cathepsin D) was assayed from m. rectus femoris of mice trained 5 days per week, 1 hr per day for 4 weeks according to 4 different programmes: I. running speed 20 m/min, horizontal track, II. 25 m/min, horizontal track, III. 20 m/min 8 degrees uphill inclination, and IV. 25 m/min 8 degrees uphill inclination. Oxidative capacity increased and anaerobic capacity decreased without distinction between the different traning programmes. Of acid hydrolases assayed the activities of beta-glucuronidase and cathepsin D were increased independently of training intensity. Simultaneous histochemical observations on beta-glucuronidase and arylsulphatase activities in the contralateral m. rectus femoris showed more intense staining in red as compared to white muscle fibres. It is suggested that training affected the red fibres and that the applied level of loading was probably too low to cause major involvement of white fibres.
对每周训练5天、每天训练1小时、持续4周的小鼠股直肌，依据4种不同方案测定了能量代谢的某些酶（细胞色素c氧化酶、柠檬酸合酶、苹果酸脱氢酶和乳酸脱氢酶）以及溶酶体（β-葡萄糖醛酸酶、β-N-乙酰氨基葡萄糖苷酶、芳基硫酸酯酶、核糖核酸酶、脱氧核糖核酸酶、酸性磷酸酶和组织蛋白酶D）的活性：方案I. 跑步速度20米/分钟，水平跑道；方案II. 25米/分钟，水平跑道；方案III. 20米/分钟，8度上坡倾斜；方案IV. 25米/分钟，8度上坡倾斜。氧化能力增强，无氧能力降低，不同训练方案之间无差异。在所测定的酸性水解酶中，β-葡萄糖醛酸酶和组织蛋白酶D的活性增加，且与训练强度无关。对股直肌对侧的β-葡萄糖醛酸酶和芳基硫酸酯酶活性进行的同步组织化学观察显示，与白色肌纤维相比，红色肌纤维的染色更强。提示训练影响了红色肌纤维，且所施加的负荷水平可能过低，不足以引起白色肌纤维的主要参与。
