Georgi A, Mottola-Hartshorn C, Warner A, Fields B, Chen L B
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA.
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6579-83. doi: 10.1073/pnas.87.17.6579.
Reovirus serotype 1 (Lang) can be conjugated with rhodamine B or fluorescein isothiocyanate in a way that preserves viral infectivity. We have used epifluorescence microscopy to detect individual virions bound to the surface of cells and to follow in real time the early stages of reovirus infection in living cells. Following uptake of the virus into endocytic vesicles, the movement of these vesicles can be observed readily. The vesicle movement is inhibited by nocodazole or colchicine, consistent with previous findings that the movement of intracellular vesicles is often microtubule-based.
呼肠孤病毒1型（朗株）能够以保持病毒感染性的方式与罗丹明B或异硫氰酸荧光素结合。我们利用落射荧光显微镜检测与细胞表面结合的单个病毒粒子，并实时追踪活细胞中呼肠孤病毒感染的早期阶段。病毒被摄入内吞小泡后，这些小泡的移动很容易被观察到。小泡的移动受到诺考达唑或秋水仙碱的抑制，这与之前关于细胞内小泡移动通常基于微管的发现一致。
