槟榔对雌性大鼠的抗排卵和堕胎作用。
Antiovulatory and abortifacient effects of Areca catechu (betel nut) in female rats.
机构信息
Department of Pharmacology, Kasturba Medical College, Manipal, Manipal University, Karnataka, India.
出版信息
Indian J Pharmacol. 2010 Oct;42(5):306-11. doi: 10.4103/0253-7613.70350.
OBJECTIVES
To study the antiovulatory and abortifacient effects of ethanolic extract of Areca catechu in female rats.
MATERIALS AND METHODS
For antiovulatory effect, ethanolic extract of A. catechu at 100 and 300 mg/kg doses was administered orally for 15 days. Vaginal smears were examined daily microscopically for estrus cycle. Rats were sacrificed on 16(th) day. Ovarian weight, cholesterol estimation, and histopathological studies were done. Abortifacient activity was studied in rats at 100 and 300 mg/kg doses administered orally from 6(th) to 15(th) day of pregnancy. Rats were laparotomised on 19(th) day. The number of implantation sites and live fetuses were observed in both horns of the uterus.
RESULTS
The extract of A. catechu showed a significant decrease in the duration of estrus at 100 mg/kg (P = 0.015) and 300 mg/kg doses (P = 0.002) as compared with control. Metestrus phase was also significantly reduced at 100 mg/kg (P = 0.024) and 300 mg/kg doses (P = 0.002). There was a significant increase in proestrus (P < 0.001) phase. However, diestrus phase was unchanged. Histopathological study of the ovaries showed mainly primordial, primary, and secondary follicles in the test groups as compared to control. There was also a significant (P = 0.002) decrease in ovarian weight and a significant (P = 0.021) increase in ovarian cholesterol level at 100 mg/kg dose. In the study to evaluate abortifacient effect, the mean percentage of abortion with 100 and 300 mg/kg doses were 75.5% and 72.22%, respectively, which was significantly (P = 0.008 and P = 0.006, respectively) increased when compared with control.
CONCLUSION
The ethanolic extract of A. catechu at doses of 100 and 300 mg/kg has antiovulatory and abortifacient effects.
目的
研究槟榔乙醇提取物对雌性大鼠的抗排卵和堕胎作用。
材料和方法
为了研究抗排卵作用，将槟榔乙醇提取物以 100 和 300 mg/kg 的剂量经口给予 15 天。每天用显微镜检查阴道涂片以确定动情周期。第 16 天处死大鼠。测定卵巢重量、胆固醇含量，并进行组织病理学研究。在妊娠第 6 至 15 天，以 100 和 300 mg/kg 的剂量经口给予槟榔乙醇提取物，研究其堕胎活性。第 19 天剖腹术，观察子宫两侧的着床部位和活胎数。
结果
与对照组相比，槟榔乙醇提取物 100 mg/kg（P=0.015）和 300 mg/kg（P=0.002）剂量可显著缩短动情期持续时间。100 mg/kg（P=0.024）和 300 mg/kg（P=0.002）剂量也可显著减少动情前期。动情前期明显增加（P<0.001）。然而，动情后期不变。卵巢组织病理学研究显示，与对照组相比，实验组主要为原始卵泡、初级卵泡和次级卵泡。100 mg/kg 剂量组卵巢重量显著降低（P=0.002），卵巢胆固醇水平显著升高（P=0.021）。在评估堕胎作用的研究中，100 和 300 mg/kg 剂量的平均流产率分别为 75.5%和 72.22%，与对照组相比显著增加（分别为 P=0.008 和 P=0.006）。
结论
槟榔乙醇提取物剂量为 100 和 300 mg/kg 具有抗排卵和堕胎作用。
Zotero 插件