Laboratory of Microbiology of GNU Medical School, Gyeongsang National University, 900 Gajwa-Dong, Chinju, Korea.
Biotechnol Lett. 2011 May;33(5):921-7. doi: 10.1007/s10529-010-0509-0. Epub 2011 Jan 5.
The concentration of cardiac troponin I (cTnI) in blood is an important marker for heart muscle cell damage. A surface plasmon resonance (SPR)-based immunosensor was devised for the rapid and specific detection of cTnI. It was constructed by crosslinking a monoclonal antibody P-II-13, which was generated against a loop region (aa 84-94) of cTnI protein as an epitope peptide, onto a chemically modified thin gold film. The performance of the sensor was examined with respect to the SPR signal intensity versus cTnI concentration. The signal intensity was directly correlated with the cTnI concentration in the range of 0-160 μg/l. The sensor signal was saturated when the concentration of cTnI approached 660 μg/l with the SPR intensity of 172 RU. The lower detection limit of the sensor was 68 ng/l cTnI, which was comparable to ELISA-based commercial cTnI detection systems.
血液中心肌肌钙蛋白 I（cTnI）的浓度是心肌细胞损伤的重要标志物。设计了一种基于表面等离子体共振（SPR）的免疫传感器，用于快速和特异性检测 cTnI。它是通过将针对 cTnI 蛋白的一个环区（aa84-94）作为表位肽产生的单克隆抗体 P-II-13 交联到化学修饰的薄金膜上来构建的。传感器的性能是根据 SPR 信号强度与 cTnI 浓度之间的关系来检查的。信号强度与 cTnI 浓度在 0-160μg/l 的范围内呈直接相关。当 cTnI 浓度接近 660μg/l 时，传感器信号饱和，SPR 强度为 172RU。传感器的检测下限为 68ng/l cTnI，与基于 ELISA 的商业 cTnI 检测系统相当。
