C.H.U. de Bordeaux, Hôpital Pellegrin, Laboratoire de Bactériologie, 33076 Bordeaux Cedex, France.
J Clin Microbiol. 2011 Mar;49(3):941-4. doi: 10.1128/JCM.01489-10. Epub 2011 Jan 5.
Campylobacter species, especially Campylobacter jejuni and Campylobacter coli, are a major cause of human bacterial enteritis. Current detection in stools is done essentially by culture on selective and nonselective media with filtration. These methods were compared to 2 molecular biology methods, an in-house real-time PCR and a multiplex PCR named Seeplex Diarrhea ACE Detection, and 3 immunoenzymatic methods, Premier Campy, RidaScreen Campylobacter, and ImmunoCard Stat!Campy. Out of 242 stool specimens tested, 23 (9.5%) fulfilled the positivity criteria, i.e., they were positive by one or both culture methods or, in case of a negative culture, by a positive molecular method and a positive immunoenzymatic method. The striking feature of this study is the low sensitivity of culture, in the range of 60%, in contrast to immunoenzymatic and molecular tests.
弯曲菌属，尤其是空肠弯曲菌和大肠弯曲菌，是人类细菌性肠炎的主要致病菌。目前，粪便中的检测主要通过选择性和非选择性培养基结合过滤进行培养。这些方法与 2 种分子生物学方法（一种内部实时 PCR 和一种称为 Seeplex Diarrhea ACE Detection 的多重 PCR）和 3 种免疫酶方法（Premier Campy、RidaScreen Campylobacter 和 ImmunoCard Stat!Campy）进行了比较。在 242 份粪便标本中，有 23 份（9.5%）符合阳性标准，即它们通过一种或两种培养方法呈阳性，或者在培养呈阴性的情况下，通过一种阳性的分子方法和一种阳性的免疫酶方法呈阳性。这项研究的显著特点是培养的敏感性较低，范围在 60%，与免疫酶和分子检测相比。
