Department of Chemistry, Liaoning University, Shenyang, PR China.
Ultrason Sonochem. 2011 Sep;18(5):1028-34. doi: 10.1016/j.ultsonch.2010.12.006. Epub 2010 Dec 30.
In this paper, in order to examine the mechanisms of sonodynamic and photodynamic reactions, the chlorophyllin metal (Chl-M (M=Fe, Mg and Cu)) complexes were irradiated by ultrasound (US) and visible-light (VL), respectively, and the generation of reactive oxygen species (ROS) were detected by the method of Oxidation-Extraction Spectrometry (OES). That is, the 1,5-diphenyl carbazide (DPCI) is oxidized by the generated ROS into 1,5-diphenyl carbazone (DPCO), which can display a various visible absorption around 563 nm wavelength. Besides, some influence parameters on the generation of ROS were also reviewed. The results demonstrated an apparent synergistic effect of Chl-M and ultrasonic or visible-light irradiation for the generation of ROS. Moreover, the quantities of generated ROS increase with the increase of (ultrasonic or visible-light) irradiation time and Chl-M (M=Fe, Mg and Cu) concentration. Finally, several quenchers were used to determine the kind of the generated ROS. It is wished that this paper might offer some valuable references for the study on the sonodynamic therapy (SDT) and photodynamic therapy (PDT) mechanisms and the application of Chl-M in tumor treatment.
在本文中，为了研究声动力学和光动力学反应的机制，分别用超声（US）和可见光（VL）辐照叶绿素金属（Chl-M（M=Fe、Mg 和 Cu））配合物，并通过氧化萃取光谱法（OES）检测活性氧（ROS）的生成。也就是说，生成的 ROS 将 1,5-二苯基卡巴肼（DPCI）氧化成 1,5-二苯基卡巴腙（DPCO），它可以在 563nm 波长左右显示出各种可见吸收。此外，还考察了一些影响 ROS 生成的参数。结果表明，Chl-M 与超声或可见光辐照的协同作用明显有利于 ROS 的生成。而且，ROS 的生成量随着（超声或可见光）辐照时间和 Chl-M（M=Fe、Mg 和 Cu）浓度的增加而增加。最后，使用几种猝灭剂来确定生成的 ROS 的种类。希望本文能为声动力学治疗（SDT）和光动力学治疗（PDT）机制的研究以及 Chl-M 在肿瘤治疗中的应用提供一些有价值的参考。
