Center for Biofunctional Molecules, Department of Chemistry, Yonsei University, Seoul 120-749, Korea.
Chem Soc Rev. 2011 May;40(5):2120-30. doi: 10.1039/c0cs00118j. Epub 2011 Jan 14.
Fluorescent probes have been used extensively to monitor biomolecules and biologically relevant species in vitro and in vivo. A new trend in this area that has been stimulated by the desire to obtain more detailed information about the biological effects of analytes is the change from live cell to whole animal fluorescent imaging. Zebrafish has received great attention for live vertebrate imaging due to several noticeable advantages. In this tutorial review, recent advances in live zebrafish imaging using fluorescent probes, such as fluorescent proteins, synthetic fluorescent dyes and quantum dots, are highlighted.
荧光探针已广泛用于体外和体内监测生物分子和与生物相关的物种。由于人们渴望获得关于分析物生物效应的更详细信息，该领域出现了一个新趋势，即将荧光成像从活细胞转变为整个动物。由于具有几个显著的优点，斑马鱼成为活体脊椎动物成像的热门选择。在本综述教程中，重点介绍了使用荧光探针（如荧光蛋白、合成荧光染料和量子点）进行活体斑马鱼成像的最新进展。
