Debiaggi M, Tateo F, Pagani L, Luini M, Romero E
Istituto di Microbiologia, Università degli Studi di Pavia, Italy.
Microbiologica. 1990 Jul;13(3):207-13.
The effect of five propolis flavonoids on the infectivity and replication of some herpesvirus, adenovirus, coronavirus and rotavirus strains has been studied. Experiments were performed in vitro in cell cultures using the viral plaque reduction technique. The cytotoxicity of flavonoids, including chrysine, kaempferol, acacetin, galangin and quercetin, was evaluated on uninfected monolayers to determine their effect on cell growth and viability. Chrysine and kaempferol caused a concentration-dependent reduction of intracellular replication of herpes-virus strains when monolayers were infected and subsequently cultured in a drug-containing medium. However, virus infectivity was not significantly affected. Acacetin and galangin had no effect on either the infectivity or replication of any of the viruses studied. Quercetin reduced infectivity and intracellular replication, but only at the highest concentrations tested.
研究了五种蜂胶类黄酮对某些疱疹病毒、腺病毒、冠状病毒和轮状病毒株的感染性和复制的影响。使用病毒蚀斑减少技术在细胞培养物中进行体外实验。在未感染的单层细胞上评估了包括白杨素、山奈酚、刺槐素、高良姜素和槲皮素在内的类黄酮的细胞毒性，以确定它们对细胞生长和活力的影响。当单层细胞被感染并随后在含药培养基中培养时，白杨素和山奈酚引起疱疹病毒株细胞内复制的浓度依赖性降低。然而，病毒感染性没有受到显著影响。刺槐素和高良姜素对所研究的任何病毒的感染性或复制均无影响。槲皮素仅在测试的最高浓度下降低感染性和细胞内复制。
