Department of Physics, Korea University, Seoul 136-701, South Korea.
Opt Lett. 2011 Jan 15;36(2):148-50. doi: 10.1364/OL.36.000148.
We present a high-speed synthetic aperture microscopy for quantitative phase imaging of live biological cells. We measure 361 complex amplitude images of an object with various directions of illumination covering an NA of 0.8 in less than one-thirteenth of a second and then combine the images with a phase-referencing method to create a synthesized phase image. Because of the increased depth selectivity, artifacts from diffraction that are typically present in coherent imaging are significantly suppressed, and lateral resolution of phase imaging is improved. We use the instrument to demonstrate high-quality phase imaging of live cells, both static and dynamic, and thickness measurements of a nanoscale cholesterol helical ribbon.
我们提出了一种高速合成孔径显微镜，用于对活生物细胞进行定量相位成像。我们在不到三分之一秒的时间内用不同方向的照明测量了物体的 361 个复振幅图像，覆盖了 0.8 的 NA，并使用相位参考方法将图像组合起来生成合成相位图像。由于深度选择性的提高，通常在相干成像中存在的来自衍射的伪影被显著抑制，并且相位成像的横向分辨率得到提高。我们使用该仪器演示了活细胞的高质量静态和动态相位成像，以及纳米级胆固醇螺旋带的厚度测量。
