Graf Benedikt W, Ralston Tyler S, Ko Han-Jo, Boppart Stephen A
Department of Electrical and Computer Engineering, Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign405 N. Mathews Avenue, Urbana, IL 61801, USA.
Opt Express. 2009 Aug 3;17(16):13447-57. doi: 10.1364/oe.17.013447.
We demonstrate how optical coherence imaging techniques can detect intrinsic scattering changes that occur during action potentials in single neurons. Using optical coherence tomography (OCT), an increase in scattering intensity from neurons in the abdominal ganglion of Aplysia californica is observed following electrical stimulation of the connective nerve. In addition, optical coherence microscopy (OCM), with its superior transverse spatial resolution, is used to demonstrate a direct correlation between scattering intensity changes and membrane voltage in single cultured Aplysia bag cell neurons during evoked action potentials. While intrinsic scattering changes are small, OCT and OCM have potential use as tools in neuroscience research for non-invasive and non-contact measurement of neural activity without electrodes or fluorescent dyes. These techniques have many attractive features such as high sensitivity and deep imaging penetration depth, as well as high temporal and spatial resolution. This study demonstrates the first use of OCT and OCM to detect functionally-correlated optical scattering changes in single neurons.
我们展示了光学相干成像技术如何检测单个神经元动作电位期间发生的内在散射变化。使用光学相干断层扫描（OCT），在对加州海兔腹神经节的神经元进行电刺激后，观察到散射强度增加。此外，具有卓越横向空间分辨率的光学相干显微镜（OCM）被用于证明在诱发动作电位期间，单个培养的加州海兔袋状细胞神经元的散射强度变化与膜电压之间存在直接关联。虽然内在散射变化很小，但OCT和OCM有潜力作为神经科学研究工具，用于在无需电极或荧光染料的情况下对神经活动进行非侵入性和非接触式测量。这些技术具有许多吸引人的特性，如高灵敏度、深成像穿透深度以及高时间和空间分辨率。本研究首次展示了使用OCT和OCM来检测单个神经元中与功能相关的光学散射变化。
