Department of Basic Health Sciences, University Rey Juan Carlos (URJC), 28922 Alcorcón, Spain.
High Performance Research Group in Physiopathology and Pharmacology of the Digestive System (NeuGut-URJC), University Rey Juan Carlos, 28922 Alcorcón, Spain.
Int J Environ Res Public Health. 2022 May 26;19(11):6485. doi: 10.3390/ijerph19116485.
Gastrointestinal pathologies associated with abdominal pain, such as irritable bowel syndrome or inflammatory bowel disease, lack sufficiently effective treatments. In our study we have used a rat model of visceral pain (72 animals; = 8-13 per experimental group) to analyze the consequences of intracolonic administration of the irritant acetic acid on visceral sensitivity, histology of the colonic wall, and inflammatory response. Moreover, we have studied the possible beneficial effects of a pretreatment with a commercial probiotic (Actimel). Contrary to expectations, acetic acid application (7 cm proximal to the anus) decreased the nociceptive response to intracolonic mechanical stimulation, with a slight increase in the histological damage of colonic mucosa. The intensity of these changes depended on the concentration (4% or 0.6%) and the time of application (30 or 60 min). Pretreatment with probiotics (by daily gavage, for 1 week) normalized the values obtained in the visceral sensitivity test but revealed an increase in the number of macrophages. These results suggest a possible activation of inhibitory mechanisms early after colonic irritation, not previously described (which need further experimental confirmation), and the ability of probiotics to normalize the effects of acetic acid. In addition, pretreatment with probiotics has a direct effect on immune functions, stimulating macrophagic activity.
与腹痛相关的胃肠道病变，如肠易激综合征或炎症性肠病，缺乏足够有效的治疗方法。在我们的研究中，我们使用了内脏痛大鼠模型（72 只动物；每组 = 8-13 只），以分析将刺激性乙酸注入结肠对内脏敏感性、结肠壁组织学和炎症反应的影响。此外，我们还研究了商业益生菌（Actimel）预处理的可能有益效果。与预期相反，乙酸应用（距肛门 7cm 处）降低了对结肠内机械刺激的痛觉反应，结肠黏膜的组织损伤略有增加。这些变化的强度取决于浓度（4%或 0.6%）和应用时间（30 或 60 分钟）。益生菌预处理（通过每日灌胃，持续 1 周）使内脏敏感性测试中的值恢复正常，但增加了巨噬细胞的数量。这些结果表明，在结肠刺激后早期可能会激活以前未描述的抑制机制（需要进一步的实验证实），并且益生菌能够使乙酸的作用正常化。此外，益生菌预处理对免疫功能有直接影响，刺激巨噬细胞活性。
